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Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()

OBJECTIVES: Influenza household transmission studies are required to guide prevention strategies but most passively recruit index cases that seek healthcare. We investigated A(H1N1)pdm09 transmission in a household-based cohort during 2009. METHODS: Health-workers visited 270 households weekly, and...

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Autores principales: Thai, Pham Quang, Mai, Le Quynh, Welkers, Matthijs R.A., Hang, Nguyen Le Khanh, Thanh, Le Thi, Dung, Vu Tien Viet, Yen, Nguyen Thi Thu, Duong, Tran Nhu, Hoa, Le Nguyen Minh, Thoang, Dang Dinh, Trang, Hoang Thi Huyen, de Jong, Menno D., Wertheim, Heiman, Hien, Nguyen Tran, Horby, Peter, Fox, Annette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: W.B. Saunders 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031397/
https://www.ncbi.nlm.nih.gov/pubmed/24491598
http://dx.doi.org/10.1016/j.jinf.2014.01.008
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author Thai, Pham Quang
Mai, Le Quynh
Welkers, Matthijs R.A.
Hang, Nguyen Le Khanh
Thanh, Le Thi
Dung, Vu Tien Viet
Yen, Nguyen Thi Thu
Duong, Tran Nhu
Hoa, Le Nguyen Minh
Thoang, Dang Dinh
Trang, Hoang Thi Huyen
de Jong, Menno D.
Wertheim, Heiman
Hien, Nguyen Tran
Horby, Peter
Fox, Annette
author_facet Thai, Pham Quang
Mai, Le Quynh
Welkers, Matthijs R.A.
Hang, Nguyen Le Khanh
Thanh, Le Thi
Dung, Vu Tien Viet
Yen, Nguyen Thi Thu
Duong, Tran Nhu
Hoa, Le Nguyen Minh
Thoang, Dang Dinh
Trang, Hoang Thi Huyen
de Jong, Menno D.
Wertheim, Heiman
Hien, Nguyen Tran
Horby, Peter
Fox, Annette
author_sort Thai, Pham Quang
collection PubMed
description OBJECTIVES: Influenza household transmission studies are required to guide prevention strategies but most passively recruit index cases that seek healthcare. We investigated A(H1N1)pdm09 transmission in a household-based cohort during 2009. METHODS: Health-workers visited 270 households weekly, and collected swabs from influenza-like-illness cases. If A(H1N1)pdm09 was RT-PCR-confirmed, all household members had symptoms assessed and swabs collected daily for 10–15 days. Viral RNA was quantified and sequenced and serology performed on pre-pandemic sera. RESULTS: Index cases were detected in 20 households containing 81 people. 98.5% lacked A(H1N1)pdm09 neutralizing antibodies in pre-pandemic sera. Eleven (18.6%, 95% CI 10.7–30.4%) of 59 contacts were infected. Virus genetic diversity within households was negligible and less than between households. Index and secondary cases were distributed between mothers, daughters and sons, and had similar virus-RNA shedding and symptom dynamics. Fathers were rarely infected. Five secondary cases (45%) had no apparent symptoms and three shed virus before symptoms. Secondary infection was associated with index case wet cough (OR 1.56, 95% CI 1.22–1.99). CONCLUSIONS: In this cohort of A(H1N1)pdm09 susceptible persons, virus sequencing was capable of discriminating household from community transmission. Household transmission involved mothers and children but rarely fathers. Asymptomatic or pre-symptomatic shedding was common.
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spelling pubmed-40313972014-06-01 Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort() Thai, Pham Quang Mai, Le Quynh Welkers, Matthijs R.A. Hang, Nguyen Le Khanh Thanh, Le Thi Dung, Vu Tien Viet Yen, Nguyen Thi Thu Duong, Tran Nhu Hoa, Le Nguyen Minh Thoang, Dang Dinh Trang, Hoang Thi Huyen de Jong, Menno D. Wertheim, Heiman Hien, Nguyen Tran Horby, Peter Fox, Annette J Infect Article OBJECTIVES: Influenza household transmission studies are required to guide prevention strategies but most passively recruit index cases that seek healthcare. We investigated A(H1N1)pdm09 transmission in a household-based cohort during 2009. METHODS: Health-workers visited 270 households weekly, and collected swabs from influenza-like-illness cases. If A(H1N1)pdm09 was RT-PCR-confirmed, all household members had symptoms assessed and swabs collected daily for 10–15 days. Viral RNA was quantified and sequenced and serology performed on pre-pandemic sera. RESULTS: Index cases were detected in 20 households containing 81 people. 98.5% lacked A(H1N1)pdm09 neutralizing antibodies in pre-pandemic sera. Eleven (18.6%, 95% CI 10.7–30.4%) of 59 contacts were infected. Virus genetic diversity within households was negligible and less than between households. Index and secondary cases were distributed between mothers, daughters and sons, and had similar virus-RNA shedding and symptom dynamics. Fathers were rarely infected. Five secondary cases (45%) had no apparent symptoms and three shed virus before symptoms. Secondary infection was associated with index case wet cough (OR 1.56, 95% CI 1.22–1.99). CONCLUSIONS: In this cohort of A(H1N1)pdm09 susceptible persons, virus sequencing was capable of discriminating household from community transmission. Household transmission involved mothers and children but rarely fathers. Asymptomatic or pre-symptomatic shedding was common. W.B. Saunders 2014-06 /pmc/articles/PMC4031397/ /pubmed/24491598 http://dx.doi.org/10.1016/j.jinf.2014.01.008 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Thai, Pham Quang
Mai, Le Quynh
Welkers, Matthijs R.A.
Hang, Nguyen Le Khanh
Thanh, Le Thi
Dung, Vu Tien Viet
Yen, Nguyen Thi Thu
Duong, Tran Nhu
Hoa, Le Nguyen Minh
Thoang, Dang Dinh
Trang, Hoang Thi Huyen
de Jong, Menno D.
Wertheim, Heiman
Hien, Nguyen Tran
Horby, Peter
Fox, Annette
Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title_full Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title_fullStr Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title_full_unstemmed Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title_short Pandemic H1N1 virus transmission and shedding dynamics in index case households of a prospective Vietnamese cohort()
title_sort pandemic h1n1 virus transmission and shedding dynamics in index case households of a prospective vietnamese cohort()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031397/
https://www.ncbi.nlm.nih.gov/pubmed/24491598
http://dx.doi.org/10.1016/j.jinf.2014.01.008
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