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Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling

Ectomycorrhizal symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cycling and in functioning of the forest ecosystem. Trees with ectomycorrhizal root tips are more tolerant to environmental stresses, such as drought, and b...

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Autores principales: Sebastiana, Mónica, Vieira, Bruno, Lino-Neto, Teresa, Monteiro, Filipa, Figueiredo, Andreia, Sousa, Lisete, Pais, Maria Salomé, Tavares, Rui, Paulo, Octávio S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032270/
https://www.ncbi.nlm.nih.gov/pubmed/24859293
http://dx.doi.org/10.1371/journal.pone.0098376
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author Sebastiana, Mónica
Vieira, Bruno
Lino-Neto, Teresa
Monteiro, Filipa
Figueiredo, Andreia
Sousa, Lisete
Pais, Maria Salomé
Tavares, Rui
Paulo, Octávio S.
author_facet Sebastiana, Mónica
Vieira, Bruno
Lino-Neto, Teresa
Monteiro, Filipa
Figueiredo, Andreia
Sousa, Lisete
Pais, Maria Salomé
Tavares, Rui
Paulo, Octávio S.
author_sort Sebastiana, Mónica
collection PubMed
description Ectomycorrhizal symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cycling and in functioning of the forest ecosystem. Trees with ectomycorrhizal root tips are more tolerant to environmental stresses, such as drought, and biotic stresses such as root pathogens. Detailed information on these molecular processes is essential for the understanding of symbiotic tissue development in order to optimize the benefits of this natural phenomenon. Next generation sequencing tools allow the analysis of non model ectomycorrhizal plant-fungal interactions that can contribute to find the “symbiosis toolkits” and better define the role of each partner in the mutualistic interaction. By using 454 pyrosequencing we compared ectomycorrhizal cork oak roots with non-symbiotic roots. From the two cDNA libraries sequenced, over 2 million reads were obtained that generated 19552 cork oak root unique transcripts. A total of 2238 transcripts were found to be differentially expressed when ECM roots were compared with non-symbiotic roots. Identification of up- and down-regulated gens in ectomycorrhizal roots lead to a number of insights into the molecular mechanisms governing this important symbiosis. In cork oak roots, ectomycorrhizal colonization resulted in extensive cell wall remodelling, activation of the secretory pathway, alterations in flavonoid biosynthesis, and expression of genes involved in the recognition of fungal effectors. In addition, we identified genes with putative roles in symbiotic processes such as nutrient exchange with the fungal partner, lateral root formation or root hair decay. These findings provide a global overview of the transcriptome of an ectomycorrhizal host root, and constitute a foundation for future studies on the molecular events controlling this important symbiosis.
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spelling pubmed-40322702014-05-28 Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling Sebastiana, Mónica Vieira, Bruno Lino-Neto, Teresa Monteiro, Filipa Figueiredo, Andreia Sousa, Lisete Pais, Maria Salomé Tavares, Rui Paulo, Octávio S. PLoS One Research Article Ectomycorrhizal symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cycling and in functioning of the forest ecosystem. Trees with ectomycorrhizal root tips are more tolerant to environmental stresses, such as drought, and biotic stresses such as root pathogens. Detailed information on these molecular processes is essential for the understanding of symbiotic tissue development in order to optimize the benefits of this natural phenomenon. Next generation sequencing tools allow the analysis of non model ectomycorrhizal plant-fungal interactions that can contribute to find the “symbiosis toolkits” and better define the role of each partner in the mutualistic interaction. By using 454 pyrosequencing we compared ectomycorrhizal cork oak roots with non-symbiotic roots. From the two cDNA libraries sequenced, over 2 million reads were obtained that generated 19552 cork oak root unique transcripts. A total of 2238 transcripts were found to be differentially expressed when ECM roots were compared with non-symbiotic roots. Identification of up- and down-regulated gens in ectomycorrhizal roots lead to a number of insights into the molecular mechanisms governing this important symbiosis. In cork oak roots, ectomycorrhizal colonization resulted in extensive cell wall remodelling, activation of the secretory pathway, alterations in flavonoid biosynthesis, and expression of genes involved in the recognition of fungal effectors. In addition, we identified genes with putative roles in symbiotic processes such as nutrient exchange with the fungal partner, lateral root formation or root hair decay. These findings provide a global overview of the transcriptome of an ectomycorrhizal host root, and constitute a foundation for future studies on the molecular events controlling this important symbiosis. Public Library of Science 2014-05-23 /pmc/articles/PMC4032270/ /pubmed/24859293 http://dx.doi.org/10.1371/journal.pone.0098376 Text en © 2014 Sebastiana et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sebastiana, Mónica
Vieira, Bruno
Lino-Neto, Teresa
Monteiro, Filipa
Figueiredo, Andreia
Sousa, Lisete
Pais, Maria Salomé
Tavares, Rui
Paulo, Octávio S.
Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title_full Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title_fullStr Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title_full_unstemmed Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title_short Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling
title_sort oak root response to ectomycorrhizal symbiosis establishment: rna-seq derived transcript identification and expression profiling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032270/
https://www.ncbi.nlm.nih.gov/pubmed/24859293
http://dx.doi.org/10.1371/journal.pone.0098376
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