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CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function
Chemokines are small secreted proteins functionally involved in the immune system's regulation of lymphocyte migration across numerous mammalian species. Given its growing popularity in immunological models, we investigated the structure and function of chemokine CXCL12 protein in tree shrews....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032326/ https://www.ncbi.nlm.nih.gov/pubmed/24858548 http://dx.doi.org/10.1371/journal.pone.0098231 |
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author | Chen, Guiyuan Wang, Wei Meng, Shengke Zhang, Lichao Wang, Wenxue Jiang, Zongmin Yu, Min Cui, Qinghua Li, Meizhang |
author_facet | Chen, Guiyuan Wang, Wei Meng, Shengke Zhang, Lichao Wang, Wenxue Jiang, Zongmin Yu, Min Cui, Qinghua Li, Meizhang |
author_sort | Chen, Guiyuan |
collection | PubMed |
description | Chemokines are small secreted proteins functionally involved in the immune system's regulation of lymphocyte migration across numerous mammalian species. Given its growing popularity in immunological models, we investigated the structure and function of chemokine CXCL12 protein in tree shrews. We found that CXCL12 and its receptor CXCR4 in tree shrew had structural similarities to their homologous human proteins. Phylogenetic analysis supports the view that tree shrew is evolutionarily-close to the primates. Our results also showed that the human recombinant CXCL12 protein directly enhanced the migration of tree shrew's lymphocytes in vitro, while AMD3100 enhanced the mobilization of hematopoietic progenitor cells (HPCs) from bone marrow into peripheral blood in tree shrew in vivo. Collectively, these findings suggested that chemokines in tree shrews may play the same or similar roles as those in humans, and that the tree shrew is a viable animal model for studying human immunological diseases. |
format | Online Article Text |
id | pubmed-4032326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40323262014-05-28 CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function Chen, Guiyuan Wang, Wei Meng, Shengke Zhang, Lichao Wang, Wenxue Jiang, Zongmin Yu, Min Cui, Qinghua Li, Meizhang PLoS One Research Article Chemokines are small secreted proteins functionally involved in the immune system's regulation of lymphocyte migration across numerous mammalian species. Given its growing popularity in immunological models, we investigated the structure and function of chemokine CXCL12 protein in tree shrews. We found that CXCL12 and its receptor CXCR4 in tree shrew had structural similarities to their homologous human proteins. Phylogenetic analysis supports the view that tree shrew is evolutionarily-close to the primates. Our results also showed that the human recombinant CXCL12 protein directly enhanced the migration of tree shrew's lymphocytes in vitro, while AMD3100 enhanced the mobilization of hematopoietic progenitor cells (HPCs) from bone marrow into peripheral blood in tree shrew in vivo. Collectively, these findings suggested that chemokines in tree shrews may play the same or similar roles as those in humans, and that the tree shrew is a viable animal model for studying human immunological diseases. Public Library of Science 2014-05-23 /pmc/articles/PMC4032326/ /pubmed/24858548 http://dx.doi.org/10.1371/journal.pone.0098231 Text en © 2014 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Guiyuan Wang, Wei Meng, Shengke Zhang, Lichao Wang, Wenxue Jiang, Zongmin Yu, Min Cui, Qinghua Li, Meizhang CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title | CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title_full | CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title_fullStr | CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title_full_unstemmed | CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title_short | CXC Chemokine CXCL12 and Its Receptor CXCR4 in Tree Shrews (Tupaia belangeri): Structure, Expression and Function |
title_sort | cxc chemokine cxcl12 and its receptor cxcr4 in tree shrews (tupaia belangeri): structure, expression and function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032326/ https://www.ncbi.nlm.nih.gov/pubmed/24858548 http://dx.doi.org/10.1371/journal.pone.0098231 |
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