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Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model
Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral sq...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032327/ https://www.ncbi.nlm.nih.gov/pubmed/24858105 http://dx.doi.org/10.1371/journal.pone.0098208 |
| _version_ | 1782317631713312768 |
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| author | Granato, Daniela C. Zanetti, Mariana R. Kawahara, Rebeca Yokoo, Sami Domingues, Romênia R. Aragão, Annelize Z. Agostini, Michelle Carazzolle, Marcelo F. Vidal, Ramon O. Flores, Isadora L. Korvala, Johanna Cervigne, Nilva K. Silva, Alan R. S. Coletta, Ricardo D. Graner, Edgard Sherman, Nicholas E. Leme, Adriana F. Paes |
| author_facet | Granato, Daniela C. Zanetti, Mariana R. Kawahara, Rebeca Yokoo, Sami Domingues, Romênia R. Aragão, Annelize Z. Agostini, Michelle Carazzolle, Marcelo F. Vidal, Ramon O. Flores, Isadora L. Korvala, Johanna Cervigne, Nilva K. Silva, Alan R. S. Coletta, Ricardo D. Graner, Edgard Sherman, Nicholas E. Leme, Adriana F. Paes |
| author_sort | Granato, Daniela C. |
| collection | PubMed |
| description | Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules. |
| format | Online Article Text |
| id | pubmed-4032327 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-40323272014-05-28 Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model Granato, Daniela C. Zanetti, Mariana R. Kawahara, Rebeca Yokoo, Sami Domingues, Romênia R. Aragão, Annelize Z. Agostini, Michelle Carazzolle, Marcelo F. Vidal, Ramon O. Flores, Isadora L. Korvala, Johanna Cervigne, Nilva K. Silva, Alan R. S. Coletta, Ricardo D. Graner, Edgard Sherman, Nicholas E. Leme, Adriana F. Paes PLoS One Research Article Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules. Public Library of Science 2014-05-23 /pmc/articles/PMC4032327/ /pubmed/24858105 http://dx.doi.org/10.1371/journal.pone.0098208 Text en © 2014 Granato et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| spellingShingle | Research Article Granato, Daniela C. Zanetti, Mariana R. Kawahara, Rebeca Yokoo, Sami Domingues, Romênia R. Aragão, Annelize Z. Agostini, Michelle Carazzolle, Marcelo F. Vidal, Ramon O. Flores, Isadora L. Korvala, Johanna Cervigne, Nilva K. Silva, Alan R. S. Coletta, Ricardo D. Graner, Edgard Sherman, Nicholas E. Leme, Adriana F. Paes Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title | Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title_full | Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title_fullStr | Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title_full_unstemmed | Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title_short | Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model |
| title_sort | integrated proteomics identified up-regulated focal adhesion-mediated proteins in human squamous cell carcinoma in an orthotopic murine model |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032327/ https://www.ncbi.nlm.nih.gov/pubmed/24858105 http://dx.doi.org/10.1371/journal.pone.0098208 |
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