Cargando…
Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer
BACKGROUND: Lung cancer is one of the leading causes of deaths from cancer worldwide. Tumor virotherapy using naturally oncolytic Newcastle disease virus (NDV) has been shown to be safe and effective in preclinical studies and clinical trials. Previously, we have reported the NDV D90 strain that was...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032357/ https://www.ncbi.nlm.nih.gov/pubmed/24885546 http://dx.doi.org/10.1186/1743-422X-11-84 |
_version_ | 1782317638820560896 |
---|---|
author | Chai, Zheng Zhang, Peiyi Fu, Fang Zhang, Xueyun Liu, Ying Hu, Lihua Li, Xi |
author_facet | Chai, Zheng Zhang, Peiyi Fu, Fang Zhang, Xueyun Liu, Ying Hu, Lihua Li, Xi |
author_sort | Chai, Zheng |
collection | PubMed |
description | BACKGROUND: Lung cancer is one of the leading causes of deaths from cancer worldwide. Tumor virotherapy using naturally oncolytic Newcastle disease virus (NDV) has been shown to be safe and effective in preclinical studies and clinical trials. Previously, we have reported the NDV D90 strain that was isolated from natural source has an antiproliferative effect in human lung cancer cell line A549. METHODS AND RESULTS: In this study, we constructed a reverse genetics system based on the oncolytic NDV D90 strain and generated a recombinant NDV carrying a gene encoding enhanced green fluorescent protein (rNDV-GFP). The rescued virus rNDV-D90 and rNDV-GFP showed the similar characteristics of replication and apoptotic ability in lung cancer A549 cells, which suggested that the recombinant viruses sustained the property of tumor-selective replication and induced apoptosis of tumor cells. The athymic mice bearing implanted lung cancer were treated with the parental D90 virus, the rescued rNDV-D90 and rNDV-GFP via intratumoral injections, respectively. The results showed that the recombinant viruses as well as the parental D90 virus significantly suppressed the loss of body weight and tumor growth. CONCLUSIONS: The study provides a new platform to develop effective therapeutic agents for tumor treatment. The availability of the reverse genetics system for NDV D90 strain will make it possible to develop novel recombinant oncolytic viruses based on the NDV D90 strain for improving the efficacy of tumor treatment. |
format | Online Article Text |
id | pubmed-4032357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40323572014-05-24 Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer Chai, Zheng Zhang, Peiyi Fu, Fang Zhang, Xueyun Liu, Ying Hu, Lihua Li, Xi Virol J Research BACKGROUND: Lung cancer is one of the leading causes of deaths from cancer worldwide. Tumor virotherapy using naturally oncolytic Newcastle disease virus (NDV) has been shown to be safe and effective in preclinical studies and clinical trials. Previously, we have reported the NDV D90 strain that was isolated from natural source has an antiproliferative effect in human lung cancer cell line A549. METHODS AND RESULTS: In this study, we constructed a reverse genetics system based on the oncolytic NDV D90 strain and generated a recombinant NDV carrying a gene encoding enhanced green fluorescent protein (rNDV-GFP). The rescued virus rNDV-D90 and rNDV-GFP showed the similar characteristics of replication and apoptotic ability in lung cancer A549 cells, which suggested that the recombinant viruses sustained the property of tumor-selective replication and induced apoptosis of tumor cells. The athymic mice bearing implanted lung cancer were treated with the parental D90 virus, the rescued rNDV-D90 and rNDV-GFP via intratumoral injections, respectively. The results showed that the recombinant viruses as well as the parental D90 virus significantly suppressed the loss of body weight and tumor growth. CONCLUSIONS: The study provides a new platform to develop effective therapeutic agents for tumor treatment. The availability of the reverse genetics system for NDV D90 strain will make it possible to develop novel recombinant oncolytic viruses based on the NDV D90 strain for improving the efficacy of tumor treatment. BioMed Central 2014-05-12 /pmc/articles/PMC4032357/ /pubmed/24885546 http://dx.doi.org/10.1186/1743-422X-11-84 Text en Copyright © 2014 Chai et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Chai, Zheng Zhang, Peiyi Fu, Fang Zhang, Xueyun Liu, Ying Hu, Lihua Li, Xi Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title | Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title_full | Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title_fullStr | Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title_full_unstemmed | Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title_short | Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer |
title_sort | oncolytic therapy of a recombinant newcastle disease virus d90 strain for lung cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032357/ https://www.ncbi.nlm.nih.gov/pubmed/24885546 http://dx.doi.org/10.1186/1743-422X-11-84 |
work_keys_str_mv | AT chaizheng oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT zhangpeiyi oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT fufang oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT zhangxueyun oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT liuying oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT hulihua oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer AT lixi oncolytictherapyofarecombinantnewcastlediseasevirusd90strainforlungcancer |