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mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain

To better understand serotonin function in the primate brain, we examined the mRNA expression patterns of all the 13 members of the serotonin receptor (5HTR) family, by in situ hybridization (ISH) and the distribution of serotonergic terminations by serotonin transporter (SERT) protein immunohistoch...

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Autores principales: Shukla, Rammohan, Watakabe, Akiya, Yamamori, Tetsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032978/
https://www.ncbi.nlm.nih.gov/pubmed/24904298
http://dx.doi.org/10.3389/fncir.2014.00052
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author Shukla, Rammohan
Watakabe, Akiya
Yamamori, Tetsuo
author_facet Shukla, Rammohan
Watakabe, Akiya
Yamamori, Tetsuo
author_sort Shukla, Rammohan
collection PubMed
description To better understand serotonin function in the primate brain, we examined the mRNA expression patterns of all the 13 members of the serotonin receptor (5HTR) family, by in situ hybridization (ISH) and the distribution of serotonergic terminations by serotonin transporter (SERT) protein immunohistochemical analysis. Ten of the 13 5HTRs showed significant mRNA expressions in the marmoset brain. Our study shows several new features of the organization of serotonergic systems in the marmoset brain. (1) The thalamus expressed only a limited number of receptor subtypes compared with the cortex, hippocampus, and other subcortical regions. (2) In the cortex, there are layer-selective and area-selective mRNA expressions of 5HTRs. (3) Highly localized mRNA expressions of 5HT1F and 5HT3A were observed. (4) There was a conspicuous overlap of the mRNA expressions of receptor subtypes known to have somatodendritic localization of receptor proteins with dense serotonergic terminations in the visual cortex, the central lateral (CL) nucleus of the thalamus, the presubiculum, and the medial mammillary nucleus of the hypothalamus. This suggests a high correlation between serotonin availability and receptor expression at these locations. (5) The 5HTRs show differences in mRNA expression pattern between the marmoset and mouse cortices whereas the patterns of both the species were much similar in the hippocampus. We discuss the possible roles of 5HTRs in the marmoset brain revealed by the analysis of their overall mRNA expression patterns.
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spelling pubmed-40329782014-06-05 mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain Shukla, Rammohan Watakabe, Akiya Yamamori, Tetsuo Front Neural Circuits Neuroscience To better understand serotonin function in the primate brain, we examined the mRNA expression patterns of all the 13 members of the serotonin receptor (5HTR) family, by in situ hybridization (ISH) and the distribution of serotonergic terminations by serotonin transporter (SERT) protein immunohistochemical analysis. Ten of the 13 5HTRs showed significant mRNA expressions in the marmoset brain. Our study shows several new features of the organization of serotonergic systems in the marmoset brain. (1) The thalamus expressed only a limited number of receptor subtypes compared with the cortex, hippocampus, and other subcortical regions. (2) In the cortex, there are layer-selective and area-selective mRNA expressions of 5HTRs. (3) Highly localized mRNA expressions of 5HT1F and 5HT3A were observed. (4) There was a conspicuous overlap of the mRNA expressions of receptor subtypes known to have somatodendritic localization of receptor proteins with dense serotonergic terminations in the visual cortex, the central lateral (CL) nucleus of the thalamus, the presubiculum, and the medial mammillary nucleus of the hypothalamus. This suggests a high correlation between serotonin availability and receptor expression at these locations. (5) The 5HTRs show differences in mRNA expression pattern between the marmoset and mouse cortices whereas the patterns of both the species were much similar in the hippocampus. We discuss the possible roles of 5HTRs in the marmoset brain revealed by the analysis of their overall mRNA expression patterns. Frontiers Media S.A. 2014-05-19 /pmc/articles/PMC4032978/ /pubmed/24904298 http://dx.doi.org/10.3389/fncir.2014.00052 Text en Copyright © 2014 Shukla, Watakabe and Yamamori. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Shukla, Rammohan
Watakabe, Akiya
Yamamori, Tetsuo
mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title_full mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title_fullStr mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title_full_unstemmed mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title_short mRNA expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
title_sort mrna expression profile of serotonin receptor subtypes and distribution of serotonergic terminations in marmoset brain
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032978/
https://www.ncbi.nlm.nih.gov/pubmed/24904298
http://dx.doi.org/10.3389/fncir.2014.00052
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