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Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis

Agriculturally important grasses such as rice, maize, and sugarcane are evolutionarily distant from Arabidopsis, yet some components of the floral induction process are highly conserved. Flowering in sugarcane is an important factor that negatively affects cane yield and reduces sugar/ethanol produc...

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Autores principales: Coelho, Carla P., Minow, Mark A. A., Chalfun-Júnior, Antonio, Colasanti, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033272/
https://www.ncbi.nlm.nih.gov/pubmed/24904616
http://dx.doi.org/10.3389/fpls.2014.00221
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author Coelho, Carla P.
Minow, Mark A. A.
Chalfun-Júnior, Antonio
Colasanti, Joseph
author_facet Coelho, Carla P.
Minow, Mark A. A.
Chalfun-Júnior, Antonio
Colasanti, Joseph
author_sort Coelho, Carla P.
collection PubMed
description Agriculturally important grasses such as rice, maize, and sugarcane are evolutionarily distant from Arabidopsis, yet some components of the floral induction process are highly conserved. Flowering in sugarcane is an important factor that negatively affects cane yield and reduces sugar/ethanol production from this important perennial bioenergy crop. Comparative studies have facilitated the identification and characterization of putative orthologs of key flowering time genes in sugarcane, a complex polyploid plant whose genome has yet to be sequenced completely. Using this approach we identified phosphatidylethanolamine-binding protein (PEBP) gene family members in sugarcane that are similar to the archetypical FT and TFL1 genes of Arabidopsis that play an essential role in controlling the transition from vegetative to reproductive growth. Expression analysis of ScTFL1, which falls into the TFL1-clade of floral repressors, showed transcripts in developing leaves surrounding the shoot apex but not at the apex itself. ScFT1 was detected in immature leaves and apical regions of vegetatively growing plants and, after the floral transition, expression also occurred in mature leaves. Ectopic over-expression of ScTFL1 in Arabidopsis caused delayed flowering in Arabidopsis, as might be expected for a gene related to TFL1. In addition, lines with the latest flowering phenotype exhibited aerial rosette formation. Unexpectedly, over-expression of ScFT1, which has greatest similarity to the florigen-encoding FT, also caused a delay in flowering. This preliminary analysis of divergent sugarcane FT and TFL1 gene family members from Saccharum spp. suggests that their expression patterns and roles in the floral transition has diverged from the predicted role of similar PEBP family members.
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spelling pubmed-40332722014-06-05 Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis Coelho, Carla P. Minow, Mark A. A. Chalfun-Júnior, Antonio Colasanti, Joseph Front Plant Sci Plant Science Agriculturally important grasses such as rice, maize, and sugarcane are evolutionarily distant from Arabidopsis, yet some components of the floral induction process are highly conserved. Flowering in sugarcane is an important factor that negatively affects cane yield and reduces sugar/ethanol production from this important perennial bioenergy crop. Comparative studies have facilitated the identification and characterization of putative orthologs of key flowering time genes in sugarcane, a complex polyploid plant whose genome has yet to be sequenced completely. Using this approach we identified phosphatidylethanolamine-binding protein (PEBP) gene family members in sugarcane that are similar to the archetypical FT and TFL1 genes of Arabidopsis that play an essential role in controlling the transition from vegetative to reproductive growth. Expression analysis of ScTFL1, which falls into the TFL1-clade of floral repressors, showed transcripts in developing leaves surrounding the shoot apex but not at the apex itself. ScFT1 was detected in immature leaves and apical regions of vegetatively growing plants and, after the floral transition, expression also occurred in mature leaves. Ectopic over-expression of ScTFL1 in Arabidopsis caused delayed flowering in Arabidopsis, as might be expected for a gene related to TFL1. In addition, lines with the latest flowering phenotype exhibited aerial rosette formation. Unexpectedly, over-expression of ScFT1, which has greatest similarity to the florigen-encoding FT, also caused a delay in flowering. This preliminary analysis of divergent sugarcane FT and TFL1 gene family members from Saccharum spp. suggests that their expression patterns and roles in the floral transition has diverged from the predicted role of similar PEBP family members. Frontiers Media S.A. 2014-05-26 /pmc/articles/PMC4033272/ /pubmed/24904616 http://dx.doi.org/10.3389/fpls.2014.00221 Text en Copyright © 2014 Coelho, Minow, Chalfun-Júnior and Colasanti. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Coelho, Carla P.
Minow, Mark A. A.
Chalfun-Júnior, Antonio
Colasanti, Joseph
Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title_full Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title_fullStr Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title_full_unstemmed Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title_short Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis
title_sort putative sugarcane ft/tfl1 genes delay flowering time and alter reproductive architecture in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033272/
https://www.ncbi.nlm.nih.gov/pubmed/24904616
http://dx.doi.org/10.3389/fpls.2014.00221
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