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Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line

Icaritin (ICT), a hydrolytic product of icariin from Epimedium genus, exhibits antitumor activities in several human solid-tumor and myeloid leukemia cells with extensive influence on various cell signal molecules, such as MAPKs being involved in cell proliferation and Bcl-2 participating in cell ap...

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Autores principales: Li, Zi-Jian, Yao, Can, Liu, Su-Fang, Chen, Long, Xi, Ya-Ming, Zhang, Wen, Zhang, Guang-Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033340/
https://www.ncbi.nlm.nih.gov/pubmed/24895574
http://dx.doi.org/10.1155/2014/391512
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author Li, Zi-Jian
Yao, Can
Liu, Su-Fang
Chen, Long
Xi, Ya-Ming
Zhang, Wen
Zhang, Guang-Sen
author_facet Li, Zi-Jian
Yao, Can
Liu, Su-Fang
Chen, Long
Xi, Ya-Ming
Zhang, Wen
Zhang, Guang-Sen
author_sort Li, Zi-Jian
collection PubMed
description Icaritin (ICT), a hydrolytic product of icariin from Epimedium genus, exhibits antitumor activities in several human solid-tumor and myeloid leukemia cells with extensive influence on various cell signal molecules, such as MAPKs being involved in cell proliferation and Bcl-2 participating in cell apoptosis. However, the effect of icaritin on Burkitt Lymphoma has not been elucidated. In the present study, we first screened the potential effect of icaritin on Burkitt lymphoma Raji and P3HR-1 cell lines and found that icaritin showed cytotoxicity in both cell lines. We further found that icaritin could significantly inhibit Raji cells proliferation with S-phase arrest of cell cycle and induced cell apoptosis accompanied by activation of caspase-8 and caspase-9 and cleavage of PARP. We also observed that icaritin was able to decrease Bcl-2 levels, thus shifting the Bcl-2/Bax ratio, and it could obviously reduce c-Myc, a specific molecular target in Burkitt lymphoma. Our findings demonstrated that icaritin showed cytotoxicity, inhibited cell growth, caused S arrest, and induced apoptosis in Burkitt lymphoma cells and provided a rationale for the further evaluation of icaritin for Burkitt lymphoma therapy.
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spelling pubmed-40333402014-06-03 Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line Li, Zi-Jian Yao, Can Liu, Su-Fang Chen, Long Xi, Ya-Ming Zhang, Wen Zhang, Guang-Sen Biomed Res Int Research Article Icaritin (ICT), a hydrolytic product of icariin from Epimedium genus, exhibits antitumor activities in several human solid-tumor and myeloid leukemia cells with extensive influence on various cell signal molecules, such as MAPKs being involved in cell proliferation and Bcl-2 participating in cell apoptosis. However, the effect of icaritin on Burkitt Lymphoma has not been elucidated. In the present study, we first screened the potential effect of icaritin on Burkitt lymphoma Raji and P3HR-1 cell lines and found that icaritin showed cytotoxicity in both cell lines. We further found that icaritin could significantly inhibit Raji cells proliferation with S-phase arrest of cell cycle and induced cell apoptosis accompanied by activation of caspase-8 and caspase-9 and cleavage of PARP. We also observed that icaritin was able to decrease Bcl-2 levels, thus shifting the Bcl-2/Bax ratio, and it could obviously reduce c-Myc, a specific molecular target in Burkitt lymphoma. Our findings demonstrated that icaritin showed cytotoxicity, inhibited cell growth, caused S arrest, and induced apoptosis in Burkitt lymphoma cells and provided a rationale for the further evaluation of icaritin for Burkitt lymphoma therapy. Hindawi Publishing Corporation 2014 2014-05-07 /pmc/articles/PMC4033340/ /pubmed/24895574 http://dx.doi.org/10.1155/2014/391512 Text en Copyright © 2014 Zi-Jian Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Zi-Jian
Yao, Can
Liu, Su-Fang
Chen, Long
Xi, Ya-Ming
Zhang, Wen
Zhang, Guang-Sen
Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title_full Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title_fullStr Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title_full_unstemmed Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title_short Cytotoxic Effect of Icaritin and Its Mechanisms in Inducing Apoptosis in Human Burkitt Lymphoma Cell Line
title_sort cytotoxic effect of icaritin and its mechanisms in inducing apoptosis in human burkitt lymphoma cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033340/
https://www.ncbi.nlm.nih.gov/pubmed/24895574
http://dx.doi.org/10.1155/2014/391512
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