Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase

Malate dehydrogenase (MDH) plays important metabolic roles in bacteria. In this study, the recombinant MDH protein (His-MDH) of Brucella abortus was purified and its ability to catalyze the conversion of oxaloacetate (OAA) to L-malate (hereon referred to as MDH activity) was analyzed. Michaelis Cons...

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Detalles Bibliográficos
Autores principales: Han, Xiangan, Tong, Yongliang, Tian, Mingxing, Zhang, Yuxi, Sun, Xiaoqing, Wang, Shaohui, Qiu, Xusheng, Ding, Chan, Yu, Shengqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033508/
https://www.ncbi.nlm.nih.gov/pubmed/24895685
http://dx.doi.org/10.1155/2014/973751

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