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Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new char...

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Detalles Bibliográficos
Autores principales: Mahmad, Noraini, Mat Taha, Rosna, Othman, Rashidi, Saleh, Azani, Hasbullah, Nor Azlina, Elias, Hashimah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033544/
https://www.ncbi.nlm.nih.gov/pubmed/24895660
http://dx.doi.org/10.1155/2014/745148
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author Mahmad, Noraini
Mat Taha, Rosna
Othman, Rashidi
Saleh, Azani
Hasbullah, Nor Azlina
Elias, Hashimah
author_facet Mahmad, Noraini
Mat Taha, Rosna
Othman, Rashidi
Saleh, Azani
Hasbullah, Nor Azlina
Elias, Hashimah
author_sort Mahmad, Noraini
collection PubMed
description In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions.
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spelling pubmed-40335442014-06-03 Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Saleh, Azani Hasbullah, Nor Azlina Elias, Hashimah ScientificWorldJournal Research Article In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions. Hindawi Publishing Corporation 2014 2014-05-07 /pmc/articles/PMC4033544/ /pubmed/24895660 http://dx.doi.org/10.1155/2014/745148 Text en Copyright © 2014 Noraini Mahmad et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mahmad, Noraini
Mat Taha, Rosna
Othman, Rashidi
Saleh, Azani
Hasbullah, Nor Azlina
Elias, Hashimah
Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title_full Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title_fullStr Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title_full_unstemmed Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title_short Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
title_sort effects of naa and bap, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera gaertn. (lotus), an aquatic edible plant
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033544/
https://www.ncbi.nlm.nih.gov/pubmed/24895660
http://dx.doi.org/10.1155/2014/745148
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