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Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio
Background. Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities. Objectives. The aim of this study was to investigate the potential effect of HES treat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033591/ https://www.ncbi.nlm.nih.gov/pubmed/24891765 http://dx.doi.org/10.1155/2014/393217 |
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author | Abuelsaad, Abdelaziz S. A. Allam, Gamal Al-Solumani, Adnan A. A. |
author_facet | Abuelsaad, Abdelaziz S. A. Allam, Gamal Al-Solumani, Adnan A. A. |
author_sort | Abuelsaad, Abdelaziz S. A. |
collection | PubMed |
description | Background. Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities. Objectives. The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced by A. hydrophila infection in murine. Methods. A. hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks. Phagocytosis, reactive oxygen species production, CD4(+)/CD8(+) T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated. The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated. Results. Percentage of CD4(+) T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8(+) T cells percentage, and CD14 expression on monocytes were significantly reduced. On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage. Conclusion. Present data indicated that HES has a potential role in the suppression of inflammatory response induced by A. hydrophila toxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4(+)/CD8(+) cell ratio. |
format | Online Article Text |
id | pubmed-4033591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-40335912014-06-02 Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio Abuelsaad, Abdelaziz S. A. Allam, Gamal Al-Solumani, Adnan A. A. Mediators Inflamm Research Article Background. Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities. Objectives. The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced by A. hydrophila infection in murine. Methods. A. hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks. Phagocytosis, reactive oxygen species production, CD4(+)/CD8(+) T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated. The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated. Results. Percentage of CD4(+) T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8(+) T cells percentage, and CD14 expression on monocytes were significantly reduced. On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage. Conclusion. Present data indicated that HES has a potential role in the suppression of inflammatory response induced by A. hydrophila toxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4(+)/CD8(+) cell ratio. Hindawi Publishing Corporation 2014 2014-05-06 /pmc/articles/PMC4033591/ /pubmed/24891765 http://dx.doi.org/10.1155/2014/393217 Text en Copyright © 2014 Abdelaziz S. A. Abuelsaad et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Abuelsaad, Abdelaziz S. A. Allam, Gamal Al-Solumani, Adnan A. A. Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title | Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title_full | Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title_fullStr | Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title_full_unstemmed | Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title_short | Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4(+)/CD8(+) T Cell Ratio |
title_sort | hesperidin inhibits inflammatory response induced by aeromonas hydrophila infection and alters cd4(+)/cd8(+) t cell ratio |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033591/ https://www.ncbi.nlm.nih.gov/pubmed/24891765 http://dx.doi.org/10.1155/2014/393217 |
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