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Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study

BACKGROUND AND OBJECTIVES: Various bacterial species from subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. The aim of this study was to detect the presence of Tannerella forsythia (Tf) in subgingival plaque of periodontally healthy subj...

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Autores principales: Bankur, Praveen Kumar, Nayak, Aarati, Bhat, Kishore, Bankur, Rashmi, Naik, Reshma, Rajpoot, Nami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033879/
https://www.ncbi.nlm.nih.gov/pubmed/24872621
http://dx.doi.org/10.4103/0972-124X.131312
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author Bankur, Praveen Kumar
Nayak, Aarati
Bhat, Kishore
Bankur, Rashmi
Naik, Reshma
Rajpoot, Nami
author_facet Bankur, Praveen Kumar
Nayak, Aarati
Bhat, Kishore
Bankur, Rashmi
Naik, Reshma
Rajpoot, Nami
author_sort Bankur, Praveen Kumar
collection PubMed
description BACKGROUND AND OBJECTIVES: Various bacterial species from subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. The aim of this study was to detect the presence of Tannerella forsythia (Tf) in subgingival plaque of periodontally healthy subjects and chronic periodontitis patients by using both culture and PCR technique and compare the two techniques. MATERIALS AND METHODS: Pooled subgingival plaque samples were taken using sterile curettes from predetermined sites in 50 periodontally healthy subjects and from 50 periodontitis subjects. Samples were analyzed for the presence of T. forsythia using both techniques. Statistical analysis of the results was done using Chi-square test, sensitivity, and specificity tests. RESULTS: Both techniques could detect T. forsythia in subgingival plaque samples from healthy and periodontitis subjects. Periodontally healthy individuals and individuals with chronic periodontitis using the culture technique showed the presence of T. forsythia in 14 and 34%, respectively. PCR technique showed the presence of T. forsythia in 20% healthy and 40% chronic periodontitis patients. T. forsythia detection in the periodontitis group was statistically significantly higher when compared to the healthy group by both culture and PCR technique (P = 0.019 and P = 0.029). PCR demonstrated high sensitivity and low specificity when compared to the culture technique. CONCLUSION: The results indicated that T. forsythia was more prevalent in periodontitis patients when compared with healthy subjects. The PCR was found to be more sensitive than culture technique for detection of T. forsythia from the subgingival plaque samples.
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spelling pubmed-40338792014-05-28 Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study Bankur, Praveen Kumar Nayak, Aarati Bhat, Kishore Bankur, Rashmi Naik, Reshma Rajpoot, Nami J Indian Soc Periodontol Original Article BACKGROUND AND OBJECTIVES: Various bacterial species from subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. The aim of this study was to detect the presence of Tannerella forsythia (Tf) in subgingival plaque of periodontally healthy subjects and chronic periodontitis patients by using both culture and PCR technique and compare the two techniques. MATERIALS AND METHODS: Pooled subgingival plaque samples were taken using sterile curettes from predetermined sites in 50 periodontally healthy subjects and from 50 periodontitis subjects. Samples were analyzed for the presence of T. forsythia using both techniques. Statistical analysis of the results was done using Chi-square test, sensitivity, and specificity tests. RESULTS: Both techniques could detect T. forsythia in subgingival plaque samples from healthy and periodontitis subjects. Periodontally healthy individuals and individuals with chronic periodontitis using the culture technique showed the presence of T. forsythia in 14 and 34%, respectively. PCR technique showed the presence of T. forsythia in 20% healthy and 40% chronic periodontitis patients. T. forsythia detection in the periodontitis group was statistically significantly higher when compared to the healthy group by both culture and PCR technique (P = 0.019 and P = 0.029). PCR demonstrated high sensitivity and low specificity when compared to the culture technique. CONCLUSION: The results indicated that T. forsythia was more prevalent in periodontitis patients when compared with healthy subjects. The PCR was found to be more sensitive than culture technique for detection of T. forsythia from the subgingival plaque samples. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4033879/ /pubmed/24872621 http://dx.doi.org/10.4103/0972-124X.131312 Text en Copyright: © Journal of Indian Society of Periodontology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bankur, Praveen Kumar
Nayak, Aarati
Bhat, Kishore
Bankur, Rashmi
Naik, Reshma
Rajpoot, Nami
Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title_full Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title_fullStr Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title_full_unstemmed Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title_short Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
title_sort comparison of culture and polymerase chain reaction techniques in the identification of tannerella forsythia in periodontal health and disease, an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033879/
https://www.ncbi.nlm.nih.gov/pubmed/24872621
http://dx.doi.org/10.4103/0972-124X.131312
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