Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut

We investigated Candida albicans-induced mast cell degranulation in vitro and in vivo. Cell wall fraction but not culture supernatant and cell membrane fraction prepared from hyphally grown C. albicans induced β-hexosaminidase release in RBL-2H3 cells. Cell wall mannan and soluble β-glucan fractions...

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Autores principales: SAKURAI, Atsuko, YAMAGUCHI, Natsu, SONOYAMA, Kei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscience of Microbiota, Food and Health 2012
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034280/
https://www.ncbi.nlm.nih.gov/pubmed/24936351
http://dx.doi.org/10.12938/bmfh.31.67
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author SAKURAI, Atsuko
YAMAGUCHI, Natsu
SONOYAMA, Kei
author_facet SAKURAI, Atsuko
YAMAGUCHI, Natsu
SONOYAMA, Kei
author_sort SAKURAI, Atsuko
collection PubMed
description We investigated Candida albicans-induced mast cell degranulation in vitro and in vivo. Cell wall fraction but not culture supernatant and cell membrane fraction prepared from hyphally grown C. albicans induced β-hexosaminidase release in RBL-2H3 cells. Cell wall mannan and soluble β-glucan fractions also induced β-hexosaminidase release. Histological examination of mouse forestomach showed that C. albicans gut colonization induces mast cell degranulation. However, intragastric administration of cell wall fraction failed to induce mast cell degranulation. We propose that cell wall polysaccharides are responsible for mast cell degranulation in the C. albicans-colonized gut.
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spelling pubmed-40342802014-06-16 Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut SAKURAI, Atsuko YAMAGUCHI, Natsu SONOYAMA, Kei Biosci Microbiota Food Health Note We investigated Candida albicans-induced mast cell degranulation in vitro and in vivo. Cell wall fraction but not culture supernatant and cell membrane fraction prepared from hyphally grown C. albicans induced β-hexosaminidase release in RBL-2H3 cells. Cell wall mannan and soluble β-glucan fractions also induced β-hexosaminidase release. Histological examination of mouse forestomach showed that C. albicans gut colonization induces mast cell degranulation. However, intragastric administration of cell wall fraction failed to induce mast cell degranulation. We propose that cell wall polysaccharides are responsible for mast cell degranulation in the C. albicans-colonized gut. Bioscience of Microbiota, Food and Health 2012-07-26 2012 /pmc/articles/PMC4034280/ /pubmed/24936351 http://dx.doi.org/10.12938/bmfh.31.67 Text en Bioscience of Microbiota, Food and Health http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Note
SAKURAI, Atsuko
YAMAGUCHI, Natsu
SONOYAMA, Kei
Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title_full Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title_fullStr Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title_full_unstemmed Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title_short Cell Wall Polysaccharides of Candida albicans Induce Mast Cell Degranulation in the Gut
title_sort cell wall polysaccharides of candida albicans induce mast cell degranulation in the gut
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034280/
https://www.ncbi.nlm.nih.gov/pubmed/24936351
http://dx.doi.org/10.12938/bmfh.31.67
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