DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

The polymerase chain reaction (PCR) is widely applied across the biosciences, with archaeal Family-B DNA polymerases being preferred, due to their high thermostability and fidelity. The enzyme from Pyrococcus furiosus (Pfu-Pol) is more frequently used than the similar protein from Thermococcus kodak...

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Detalles Bibliográficos
Autores principales: Elshawadfy, Ashraf M., Keith, Brian J., Ee Ooi, H'Ng, Kinsman, Thomas, Heslop, Pauline, Connolly, Bernard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034419/
https://www.ncbi.nlm.nih.gov/pubmed/24904539
http://dx.doi.org/10.3389/fmicb.2014.00224

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