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Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species

BACKGROUND: The gut of phloem feeding insects is critical for nutrition uptake and xenobiotics degradation. However, partly due to its tiny size, genomic information for the gut of phloem feeding insects is limited. RESULTS: In this study, the gut transcriptomes of two species of invasive whiteflies...

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Autores principales: Ye, Xiao-Dong, Su, Yun-Lin, Zhao, Qiong-Yi, Xia, Wen-Qiang, Liu, Shu-Sheng, Wang, Xiao-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035086/
https://www.ncbi.nlm.nih.gov/pubmed/24885120
http://dx.doi.org/10.1186/1471-2164-15-370
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author Ye, Xiao-Dong
Su, Yun-Lin
Zhao, Qiong-Yi
Xia, Wen-Qiang
Liu, Shu-Sheng
Wang, Xiao-Wei
author_facet Ye, Xiao-Dong
Su, Yun-Lin
Zhao, Qiong-Yi
Xia, Wen-Qiang
Liu, Shu-Sheng
Wang, Xiao-Wei
author_sort Ye, Xiao-Dong
collection PubMed
description BACKGROUND: The gut of phloem feeding insects is critical for nutrition uptake and xenobiotics degradation. However, partly due to its tiny size, genomic information for the gut of phloem feeding insects is limited. RESULTS: In this study, the gut transcriptomes of two species of invasive whiteflies in the Bemisia tabaci complex, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), were analyzed using the Illumina sequencing. A total of 12,879 MEAM1 transcripts and 11,246 MED transcripts were annotated with a significant Blastx hit. In addition, 7,000 and 5,771 gut specific genes were respectively identified for MEAM1 and MED. Functional analyses on these gut specific genes demonstrated the important roles of gut in metabolism of insecticides and secondary plant chemicals. To reveal the molecular difference between guts of MEAM1 and MED, a comparison between gut transcriptomes of the two species was conducted and 3,910 pairs of orthologous genes were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 15 genes were found evolving under positive selection. Many of those genes are predicted to be involved in metabolism and insecticide resistance. Furthermore, many genes related to detoxification were expressed at an elevated level in the gut of MED compared to MEAM1, which might be responsible for the MED’s higher resistance to insecticides and environmental stresses. CONCLUSION: The sequencing of MED and MEAM1 gut transcriptomes and extensive comparisons of MEAM1 and MED gut transcripts provide substantial sequence information for revealing the role of gut in whiteflies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-370) contains supplementary material, which is available to authorized users.
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spelling pubmed-40350862014-06-06 Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species Ye, Xiao-Dong Su, Yun-Lin Zhao, Qiong-Yi Xia, Wen-Qiang Liu, Shu-Sheng Wang, Xiao-Wei BMC Genomics Research Article BACKGROUND: The gut of phloem feeding insects is critical for nutrition uptake and xenobiotics degradation. However, partly due to its tiny size, genomic information for the gut of phloem feeding insects is limited. RESULTS: In this study, the gut transcriptomes of two species of invasive whiteflies in the Bemisia tabaci complex, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), were analyzed using the Illumina sequencing. A total of 12,879 MEAM1 transcripts and 11,246 MED transcripts were annotated with a significant Blastx hit. In addition, 7,000 and 5,771 gut specific genes were respectively identified for MEAM1 and MED. Functional analyses on these gut specific genes demonstrated the important roles of gut in metabolism of insecticides and secondary plant chemicals. To reveal the molecular difference between guts of MEAM1 and MED, a comparison between gut transcriptomes of the two species was conducted and 3,910 pairs of orthologous genes were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 15 genes were found evolving under positive selection. Many of those genes are predicted to be involved in metabolism and insecticide resistance. Furthermore, many genes related to detoxification were expressed at an elevated level in the gut of MED compared to MEAM1, which might be responsible for the MED’s higher resistance to insecticides and environmental stresses. CONCLUSION: The sequencing of MED and MEAM1 gut transcriptomes and extensive comparisons of MEAM1 and MED gut transcripts provide substantial sequence information for revealing the role of gut in whiteflies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-370) contains supplementary material, which is available to authorized users. BioMed Central 2014-05-15 /pmc/articles/PMC4035086/ /pubmed/24885120 http://dx.doi.org/10.1186/1471-2164-15-370 Text en © Ye et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ye, Xiao-Dong
Su, Yun-Lin
Zhao, Qiong-Yi
Xia, Wen-Qiang
Liu, Shu-Sheng
Wang, Xiao-Wei
Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title_full Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title_fullStr Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title_full_unstemmed Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title_short Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
title_sort transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035086/
https://www.ncbi.nlm.nih.gov/pubmed/24885120
http://dx.doi.org/10.1186/1471-2164-15-370
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