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Silicon substrate as a novel cell culture device for myoblast cells

BACKGROUND: Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropr...

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Autores principales: Bhuyan, Mohammod K, Rodriguez-Devora, Jorge I, Fraser, Kym, Tseng, Tzu-Liang Bill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035859/
https://www.ncbi.nlm.nih.gov/pubmed/24885347
http://dx.doi.org/10.1186/1423-0127-21-47
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author Bhuyan, Mohammod K
Rodriguez-Devora, Jorge I
Fraser, Kym
Tseng, Tzu-Liang Bill
author_facet Bhuyan, Mohammod K
Rodriguez-Devora, Jorge I
Fraser, Kym
Tseng, Tzu-Liang Bill
author_sort Bhuyan, Mohammod K
collection PubMed
description BACKGROUND: Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy. RESULTS: It was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate. CONCLUSIONS: This study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.
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spelling pubmed-40358592014-05-29 Silicon substrate as a novel cell culture device for myoblast cells Bhuyan, Mohammod K Rodriguez-Devora, Jorge I Fraser, Kym Tseng, Tzu-Liang Bill J Biomed Sci Research BACKGROUND: Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy. RESULTS: It was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate. CONCLUSIONS: This study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis. BioMed Central 2014-05-16 /pmc/articles/PMC4035859/ /pubmed/24885347 http://dx.doi.org/10.1186/1423-0127-21-47 Text en Copyright © 2014 Bhuyan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Bhuyan, Mohammod K
Rodriguez-Devora, Jorge I
Fraser, Kym
Tseng, Tzu-Liang Bill
Silicon substrate as a novel cell culture device for myoblast cells
title Silicon substrate as a novel cell culture device for myoblast cells
title_full Silicon substrate as a novel cell culture device for myoblast cells
title_fullStr Silicon substrate as a novel cell culture device for myoblast cells
title_full_unstemmed Silicon substrate as a novel cell culture device for myoblast cells
title_short Silicon substrate as a novel cell culture device for myoblast cells
title_sort silicon substrate as a novel cell culture device for myoblast cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035859/
https://www.ncbi.nlm.nih.gov/pubmed/24885347
http://dx.doi.org/10.1186/1423-0127-21-47
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