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Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment

Fish farm sediments receive a large amount of organic matter from uneaten food and fecal material. This nutrient enrichment, or organic pollution, causes the accumulation of sulphide in the sediment from the action of sulphate-reducing bacteria (SRB). We investigated the effect of organic enrichment...

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Autores principales: Kondo, Ryuji, Mori, Yumi, Sakami, Tomoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036007/
https://www.ncbi.nlm.nih.gov/pubmed/22791053
http://dx.doi.org/10.1264/jsme2.ME11278
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author Kondo, Ryuji
Mori, Yumi
Sakami, Tomoko
author_facet Kondo, Ryuji
Mori, Yumi
Sakami, Tomoko
author_sort Kondo, Ryuji
collection PubMed
description Fish farm sediments receive a large amount of organic matter from uneaten food and fecal material. This nutrient enrichment, or organic pollution, causes the accumulation of sulphide in the sediment from the action of sulphate-reducing bacteria (SRB). We investigated the effect of organic enrichment around coastal fish farms comparing the SRB community structure in these sediments. Sediment samples with different levels of organic pollution classified based upon the contents of acid-volatile sulphide and chemical oxygen demand were collected at three stations on the coast of western Japan. The SRB community composition was assessed using PCR amplification, cloning, sequencing and phylogenetic analysis of the dissimilatory sulphite reductase β-subunit gene (dsrB) fragments using directly extracted sediment DNA. Sequencing of the cloned PCR products of dsrB showed the existence of different SRB groups in the sediments. The majority of dsrB sequences were associated with the families Desulfobacteraceae and Desulfobulbaceae. Clones related to the phylum Firmicutes were also detected from all sediment samples. Statistical comparison of sequences revealed that community compositions of SRB from polluted sediments significantly differed from those of moderately polluted sediments and unpolluted sediments (LIBSHUFF, p<0.05), showing a different distribution of SRB in the fish farm sediments. There is evidence showing that the organic enrichment of sediments influences the composition of SRB communities in sediments at marine fish farms.
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spelling pubmed-40360072014-07-24 Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment Kondo, Ryuji Mori, Yumi Sakami, Tomoko Microbes Environ Regular Paper Fish farm sediments receive a large amount of organic matter from uneaten food and fecal material. This nutrient enrichment, or organic pollution, causes the accumulation of sulphide in the sediment from the action of sulphate-reducing bacteria (SRB). We investigated the effect of organic enrichment around coastal fish farms comparing the SRB community structure in these sediments. Sediment samples with different levels of organic pollution classified based upon the contents of acid-volatile sulphide and chemical oxygen demand were collected at three stations on the coast of western Japan. The SRB community composition was assessed using PCR amplification, cloning, sequencing and phylogenetic analysis of the dissimilatory sulphite reductase β-subunit gene (dsrB) fragments using directly extracted sediment DNA. Sequencing of the cloned PCR products of dsrB showed the existence of different SRB groups in the sediments. The majority of dsrB sequences were associated with the families Desulfobacteraceae and Desulfobulbaceae. Clones related to the phylum Firmicutes were also detected from all sediment samples. Statistical comparison of sequences revealed that community compositions of SRB from polluted sediments significantly differed from those of moderately polluted sediments and unpolluted sediments (LIBSHUFF, p<0.05), showing a different distribution of SRB in the fish farm sediments. There is evidence showing that the organic enrichment of sediments influences the composition of SRB communities in sediments at marine fish farms. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-06 2012-02-22 /pmc/articles/PMC4036007/ /pubmed/22791053 http://dx.doi.org/10.1264/jsme2.ME11278 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Paper
Kondo, Ryuji
Mori, Yumi
Sakami, Tomoko
Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title_full Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title_fullStr Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title_full_unstemmed Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title_short Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
title_sort comparison of sulphate-reducing bacterial communities in japanese fish farm sediments with different levels of organic enrichment
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036007/
https://www.ncbi.nlm.nih.gov/pubmed/22791053
http://dx.doi.org/10.1264/jsme2.ME11278
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