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Pyrosequence Read Length of 16S rRNA Gene Affects Phylogenetic Assignment of Plant-associated Bacteria

Pyrosequence targeting of the 16S rRNA gene has been adopted for microbial communities associated with field-grown plants. To examine phylogenetic drifts according to read length and bioinformatic tools, original and chopped sequences (250–570 bp) covering the V1–V4 regions of 16S rRNA genes were co...

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Detalles Bibliográficos
Autores principales:	Okubo, Takashi, Ikeda, Seishi, Yamashita, Akifumi, Terasawa, Kimihiro, Minamisawa, Kiwamu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036018/ https://www.ncbi.nlm.nih.gov/pubmed/22791055 http://dx.doi.org/10.1264/jsme2.ME11258

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