Comparison among amoA Primers Suited for Quantification and Diversity Analyses of Ammonia-Oxidizing Bacteria in Soil

Ammonia monooxygenase subunit A gene (amoA) is frequently used as a functional gene marker for diversity analysis of ammonia-oxidizing bacteria (AOB). To select a suitable amoA primer for real-time PCR and PCR-denaturing gradient gel electrophoresis (DGGE), three reverse primers (degenerate primer a...

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Detalles Bibliográficos
Autores principales: Shimomura, Yumi, Morimoto, Sho, Hoshino, Yuko Takada, Uchida, Yoshitaka, Akiyama, Hiroko, Hayatsu, Masahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036032/
https://www.ncbi.nlm.nih.gov/pubmed/22075625
http://dx.doi.org/10.1264/jsme2.ME11230

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