Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

An anammox assay involving a (15)N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N(2) production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showe...

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Detalles Bibliográficos
Autores principales: Sato, Yoshinori, Ohta, Hiroyuki, Yamagishi, Takao, Guo, Yong, Nishizawa, Tomoyasu, Rahman, M. Habibur, Kuroda, Hisao, Kato, Task, Saito, Masanori, Yoshinaga, Ikuo, Inubushi, Kazuyuki, Suwa, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036040/
https://www.ncbi.nlm.nih.gov/pubmed/22353769
http://dx.doi.org/10.1264/jsme2.ME11330
Descripción
Sumario:An anammox assay involving a (15)N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N(2) production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus ‘Brocadia fulgida’, Ca. ‘B. anammoxidans’, and Ca. ‘Kuenenia stuttgartiensis’. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.

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