Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

An anammox assay involving a (15)N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N(2) production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showe...

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Autores principales: Sato, Yoshinori, Ohta, Hiroyuki, Yamagishi, Takao, Guo, Yong, Nishizawa, Tomoyasu, Rahman, M. Habibur, Kuroda, Hisao, Kato, Task, Saito, Masanori, Yoshinaga, Ikuo, Inubushi, Kazuyuki, Suwa, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036040/
https://www.ncbi.nlm.nih.gov/pubmed/22353769
http://dx.doi.org/10.1264/jsme2.ME11330
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author Sato, Yoshinori
Ohta, Hiroyuki
Yamagishi, Takao
Guo, Yong
Nishizawa, Tomoyasu
Rahman, M. Habibur
Kuroda, Hisao
Kato, Task
Saito, Masanori
Yoshinaga, Ikuo
Inubushi, Kazuyuki
Suwa, Yuichi
author_facet Sato, Yoshinori
Ohta, Hiroyuki
Yamagishi, Takao
Guo, Yong
Nishizawa, Tomoyasu
Rahman, M. Habibur
Kuroda, Hisao
Kato, Task
Saito, Masanori
Yoshinaga, Ikuo
Inubushi, Kazuyuki
Suwa, Yuichi
author_sort Sato, Yoshinori
collection PubMed
description An anammox assay involving a (15)N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N(2) production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus ‘Brocadia fulgida’, Ca. ‘B. anammoxidans’, and Ca. ‘Kuenenia stuttgartiensis’. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.
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spelling pubmed-40360402014-07-24 Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil Sato, Yoshinori Ohta, Hiroyuki Yamagishi, Takao Guo, Yong Nishizawa, Tomoyasu Rahman, M. Habibur Kuroda, Hisao Kato, Task Saito, Masanori Yoshinaga, Ikuo Inubushi, Kazuyuki Suwa, Yuichi Microbes Environ Short Communication An anammox assay involving a (15)N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N(2) production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus ‘Brocadia fulgida’, Ca. ‘B. anammoxidans’, and Ca. ‘Kuenenia stuttgartiensis’. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-09 2012-02-22 /pmc/articles/PMC4036040/ /pubmed/22353769 http://dx.doi.org/10.1264/jsme2.ME11330 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Sato, Yoshinori
Ohta, Hiroyuki
Yamagishi, Takao
Guo, Yong
Nishizawa, Tomoyasu
Rahman, M. Habibur
Kuroda, Hisao
Kato, Task
Saito, Masanori
Yoshinaga, Ikuo
Inubushi, Kazuyuki
Suwa, Yuichi
Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title_full Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title_fullStr Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title_full_unstemmed Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title_short Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil
title_sort detection of anammox activity and 16s rrna genes in ravine paddy field soil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036040/
https://www.ncbi.nlm.nih.gov/pubmed/22353769
http://dx.doi.org/10.1264/jsme2.ME11330
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