Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water sam...

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Detalles Bibliográficos
Autores principales: Baba, Takashi, Inoue, Naoko, Yamaguchi, Nobuyasu, Nasu, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036053/
https://www.ncbi.nlm.nih.gov/pubmed/22446304
http://dx.doi.org/10.1264/jsme2.ME11324
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author Baba, Takashi
Inoue, Naoko
Yamaguchi, Nobuyasu
Nasu, Masao
author_facet Baba, Takashi
Inoue, Naoko
Yamaguchi, Nobuyasu
Nasu, Masao
author_sort Baba, Takashi
collection PubMed
description In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-counting method. These results suggest that the microcolony method combined with fluorescent antibody staining could be useful as a monitoring technique for the prevention of Legionnaires’ disease through the early detection of L. pneumophila in freshwater.
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spelling pubmed-40360532014-07-24 Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining Baba, Takashi Inoue, Naoko Yamaguchi, Nobuyasu Nasu, Masao Microbes Environ Short Communication In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-counting method. These results suggest that the microcolony method combined with fluorescent antibody staining could be useful as a monitoring technique for the prevention of Legionnaires’ disease through the early detection of L. pneumophila in freshwater. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2012-09 2012-03-23 /pmc/articles/PMC4036053/ /pubmed/22446304 http://dx.doi.org/10.1264/jsme2.ME11324 Text en Copyright © 2012 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Baba, Takashi
Inoue, Naoko
Yamaguchi, Nobuyasu
Nasu, Masao
Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title_full Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title_fullStr Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title_full_unstemmed Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title_short Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
title_sort rapid enumeration of active legionella pneumophila in freshwater environments by the microcolony method combined with direct fluorescent antibody staining
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036053/
https://www.ncbi.nlm.nih.gov/pubmed/22446304
http://dx.doi.org/10.1264/jsme2.ME11324
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