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A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility

BACKGROUND: In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility...

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Autores principales: Wei, Shuya, Hu, Wei, Deng, Xiaomin, Zhang, Yingying, Liu, Xiaodong, Zhao, Xudong, Luo, Qingchen, Jin, Zhengyi, Li, Yin, Zhou, Shiyi, Sun, Tao, Wang, Lianzhe, Yang, Guangxiao, He, Guangyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036088/
https://www.ncbi.nlm.nih.gov/pubmed/24884869
http://dx.doi.org/10.1186/1471-2229-14-133
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author Wei, Shuya
Hu, Wei
Deng, Xiaomin
Zhang, Yingying
Liu, Xiaodong
Zhao, Xudong
Luo, Qingchen
Jin, Zhengyi
Li, Yin
Zhou, Shiyi
Sun, Tao
Wang, Lianzhe
Yang, Guangxiao
He, Guangyuan
author_facet Wei, Shuya
Hu, Wei
Deng, Xiaomin
Zhang, Yingying
Liu, Xiaodong
Zhao, Xudong
Luo, Qingchen
Jin, Zhengyi
Li, Yin
Zhou, Shiyi
Sun, Tao
Wang, Lianzhe
Yang, Guangxiao
He, Guangyuan
author_sort Wei, Shuya
collection PubMed
description BACKGROUND: In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility. RESULTS: We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes. CONCLUSIONS: Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity.
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spelling pubmed-40360882014-05-29 A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility Wei, Shuya Hu, Wei Deng, Xiaomin Zhang, Yingying Liu, Xiaodong Zhao, Xudong Luo, Qingchen Jin, Zhengyi Li, Yin Zhou, Shiyi Sun, Tao Wang, Lianzhe Yang, Guangxiao He, Guangyuan BMC Plant Biol Research Article BACKGROUND: In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility. RESULTS: We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes. CONCLUSIONS: Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity. BioMed Central 2014-05-17 /pmc/articles/PMC4036088/ /pubmed/24884869 http://dx.doi.org/10.1186/1471-2229-14-133 Text en Copyright © 2014 Wei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wei, Shuya
Hu, Wei
Deng, Xiaomin
Zhang, Yingying
Liu, Xiaodong
Zhao, Xudong
Luo, Qingchen
Jin, Zhengyi
Li, Yin
Zhou, Shiyi
Sun, Tao
Wang, Lianzhe
Yang, Guangxiao
He, Guangyuan
A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title_full A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title_fullStr A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title_full_unstemmed A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title_short A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility
title_sort rice calcium-dependent protein kinase oscpk9 positively regulates drought stress tolerance and spikelet fertility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036088/
https://www.ncbi.nlm.nih.gov/pubmed/24884869
http://dx.doi.org/10.1186/1471-2229-14-133
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