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Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer

BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growt...

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Autores principales: Chen, Jia, Wang, Tao, Zhou, Yang-Chun, Gao, Fei, Zhang, Zhi-Hong, Xu, Hao, Wang, Shou-Lin, Shen, Li-Zong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036310/
https://www.ncbi.nlm.nih.gov/pubmed/24887009
http://dx.doi.org/10.1186/1756-9966-33-38
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author Chen, Jia
Wang, Tao
Zhou, Yang-Chun
Gao, Fei
Zhang, Zhi-Hong
Xu, Hao
Wang, Shou-Lin
Shen, Li-Zong
author_facet Chen, Jia
Wang, Tao
Zhou, Yang-Chun
Gao, Fei
Zhang, Zhi-Hong
Xu, Hao
Wang, Shou-Lin
Shen, Li-Zong
author_sort Chen, Jia
collection PubMed
description BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growth factor, which can induce EMT, are able to up-regulate AQP3 expression, subsequently promoting GC cell migration and proliferation. The purpose of this study was to investigate the effects of AQP3 on EMT in human GC. METHODS: AQP3 and EMT-related proteins were detected by immunohistochemistry in human GC specimens and their clinical significance evaluated. AQP3 knockdown was attempted using small interfering RNAs, while EGF was used to up-regulate AQP3 expression. Western blotting, real-time quantitative polymerase chain reaction assays and immunofluorescence were used to evaluate changes in expression of AQP3 and EMT-related proteins in the SGC7901 and MGC803 human GC cell lines. RESULTS: AQP3 up-expression was associated with EMT-related proteins in human GC specimens, which correlated with poor prognosis for GC. AQP3 modulated GC cell proliferation, migration and invasion in vitro, and induced E-cadherin repression. AQP3 also up-regulated the expression of vimentin and fibronectin in vitro. The PI3K/AKT/SNAIL signaling pathway was likely involved in the induction of EMT by AQP3 in GC. CONCLUSIONS: AQP3 promotes EMT in human cases of GC, allowing us to understand the mechanisms of AQP3 in GC progression, thus providing a potential strategy for its treatment.
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spelling pubmed-40363102014-05-29 Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer Chen, Jia Wang, Tao Zhou, Yang-Chun Gao, Fei Zhang, Zhi-Hong Xu, Hao Wang, Shou-Lin Shen, Li-Zong J Exp Clin Cancer Res Research BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growth factor, which can induce EMT, are able to up-regulate AQP3 expression, subsequently promoting GC cell migration and proliferation. The purpose of this study was to investigate the effects of AQP3 on EMT in human GC. METHODS: AQP3 and EMT-related proteins were detected by immunohistochemistry in human GC specimens and their clinical significance evaluated. AQP3 knockdown was attempted using small interfering RNAs, while EGF was used to up-regulate AQP3 expression. Western blotting, real-time quantitative polymerase chain reaction assays and immunofluorescence were used to evaluate changes in expression of AQP3 and EMT-related proteins in the SGC7901 and MGC803 human GC cell lines. RESULTS: AQP3 up-expression was associated with EMT-related proteins in human GC specimens, which correlated with poor prognosis for GC. AQP3 modulated GC cell proliferation, migration and invasion in vitro, and induced E-cadherin repression. AQP3 also up-regulated the expression of vimentin and fibronectin in vitro. The PI3K/AKT/SNAIL signaling pathway was likely involved in the induction of EMT by AQP3 in GC. CONCLUSIONS: AQP3 promotes EMT in human cases of GC, allowing us to understand the mechanisms of AQP3 in GC progression, thus providing a potential strategy for its treatment. BioMed Central 2014-05-03 /pmc/articles/PMC4036310/ /pubmed/24887009 http://dx.doi.org/10.1186/1756-9966-33-38 Text en Copyright © 2014 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chen, Jia
Wang, Tao
Zhou, Yang-Chun
Gao, Fei
Zhang, Zhi-Hong
Xu, Hao
Wang, Shou-Lin
Shen, Li-Zong
Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title_full Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title_fullStr Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title_full_unstemmed Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title_short Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
title_sort aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036310/
https://www.ncbi.nlm.nih.gov/pubmed/24887009
http://dx.doi.org/10.1186/1756-9966-33-38
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