Cargando…
Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer
BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growt...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036310/ https://www.ncbi.nlm.nih.gov/pubmed/24887009 http://dx.doi.org/10.1186/1756-9966-33-38 |
_version_ | 1782318147217391616 |
---|---|
author | Chen, Jia Wang, Tao Zhou, Yang-Chun Gao, Fei Zhang, Zhi-Hong Xu, Hao Wang, Shou-Lin Shen, Li-Zong |
author_facet | Chen, Jia Wang, Tao Zhou, Yang-Chun Gao, Fei Zhang, Zhi-Hong Xu, Hao Wang, Shou-Lin Shen, Li-Zong |
author_sort | Chen, Jia |
collection | PubMed |
description | BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growth factor, which can induce EMT, are able to up-regulate AQP3 expression, subsequently promoting GC cell migration and proliferation. The purpose of this study was to investigate the effects of AQP3 on EMT in human GC. METHODS: AQP3 and EMT-related proteins were detected by immunohistochemistry in human GC specimens and their clinical significance evaluated. AQP3 knockdown was attempted using small interfering RNAs, while EGF was used to up-regulate AQP3 expression. Western blotting, real-time quantitative polymerase chain reaction assays and immunofluorescence were used to evaluate changes in expression of AQP3 and EMT-related proteins in the SGC7901 and MGC803 human GC cell lines. RESULTS: AQP3 up-expression was associated with EMT-related proteins in human GC specimens, which correlated with poor prognosis for GC. AQP3 modulated GC cell proliferation, migration and invasion in vitro, and induced E-cadherin repression. AQP3 also up-regulated the expression of vimentin and fibronectin in vitro. The PI3K/AKT/SNAIL signaling pathway was likely involved in the induction of EMT by AQP3 in GC. CONCLUSIONS: AQP3 promotes EMT in human cases of GC, allowing us to understand the mechanisms of AQP3 in GC progression, thus providing a potential strategy for its treatment. |
format | Online Article Text |
id | pubmed-4036310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40363102014-05-29 Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer Chen, Jia Wang, Tao Zhou, Yang-Chun Gao, Fei Zhang, Zhi-Hong Xu, Hao Wang, Shou-Lin Shen, Li-Zong J Exp Clin Cancer Res Research BACKGROUND: Gastric carcinoma (GC) is a common and lethal malignancy, and epithelial-mesenchymal transition (EMT) is believed to contribute to invasive and metastatic tumor growth. Aquaporin 3 (AQP3) is overexpressed in human GC tissues, while human epidermal growth factor (EGF) and hepatocyte growth factor, which can induce EMT, are able to up-regulate AQP3 expression, subsequently promoting GC cell migration and proliferation. The purpose of this study was to investigate the effects of AQP3 on EMT in human GC. METHODS: AQP3 and EMT-related proteins were detected by immunohistochemistry in human GC specimens and their clinical significance evaluated. AQP3 knockdown was attempted using small interfering RNAs, while EGF was used to up-regulate AQP3 expression. Western blotting, real-time quantitative polymerase chain reaction assays and immunofluorescence were used to evaluate changes in expression of AQP3 and EMT-related proteins in the SGC7901 and MGC803 human GC cell lines. RESULTS: AQP3 up-expression was associated with EMT-related proteins in human GC specimens, which correlated with poor prognosis for GC. AQP3 modulated GC cell proliferation, migration and invasion in vitro, and induced E-cadherin repression. AQP3 also up-regulated the expression of vimentin and fibronectin in vitro. The PI3K/AKT/SNAIL signaling pathway was likely involved in the induction of EMT by AQP3 in GC. CONCLUSIONS: AQP3 promotes EMT in human cases of GC, allowing us to understand the mechanisms of AQP3 in GC progression, thus providing a potential strategy for its treatment. BioMed Central 2014-05-03 /pmc/articles/PMC4036310/ /pubmed/24887009 http://dx.doi.org/10.1186/1756-9966-33-38 Text en Copyright © 2014 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Chen, Jia Wang, Tao Zhou, Yang-Chun Gao, Fei Zhang, Zhi-Hong Xu, Hao Wang, Shou-Lin Shen, Li-Zong Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title | Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title_full | Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title_fullStr | Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title_full_unstemmed | Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title_short | Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
title_sort | aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036310/ https://www.ncbi.nlm.nih.gov/pubmed/24887009 http://dx.doi.org/10.1186/1756-9966-33-38 |
work_keys_str_mv | AT chenjia aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT wangtao aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT zhouyangchun aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT gaofei aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT zhangzhihong aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT xuhao aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT wangshoulin aquaporin3promotesepithelialmesenchymaltransitioningastriccancer AT shenlizong aquaporin3promotesepithelialmesenchymaltransitioningastriccancer |