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Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients

BACKGROUND: Quaternary ammonium compounds (QAC), which contain benzalkonium chloride as the most widely used agent, are employed as wound and skin antiseptics, as well as disinfectants in hospitals. The resistance mechanism to disinfectants is usually determine by genes which are related to resistan...

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Autores principales: Mahzounieh, Mohammadreza, Khoshnood, Sheida, Ebrahimi, Azizollah, Habibian, Saeid, Yaghoubian, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: DOCS 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036384/
https://www.ncbi.nlm.nih.gov/pubmed/24872941
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author Mahzounieh, Mohammadreza
Khoshnood, Sheida
Ebrahimi, Azizollah
Habibian, Saeid
Yaghoubian, Maryam
author_facet Mahzounieh, Mohammadreza
Khoshnood, Sheida
Ebrahimi, Azizollah
Habibian, Saeid
Yaghoubian, Maryam
author_sort Mahzounieh, Mohammadreza
collection PubMed
description BACKGROUND: Quaternary ammonium compounds (QAC), which contain benzalkonium chloride as the most widely used agent, are employed as wound and skin antiseptics, as well as disinfectants in hospitals. The resistance mechanism to disinfectants is usually determine by genes which are related to resistance to quaternary ammonium compounds, namely, qacE, qacΔE1, qacΔE1 that are found in Gram-negative bacteria. OBJECTIVES: The aim of this study was to determine the incidence of antiseptic resistance genes, qacE and qacΔE1, in clinical isolates of Pseudomonas aeruginosa and Acinetobacter bumanii. MATERIALS AND METHODS: In this study, 83 clinical isolates of Pseudomonas aeruginosa, and 5 isolates of Acinetobacter baumannii from burn hospitals in Tehran and Isfahan provinces in 2010-2011, were tested by the PCR method. RESULTS: Out of the 83 clinical isolates of Pseudomonas aeruginosa, 49 isolates (50%) had the qacE gene, and 76 isolates (91.5%) had the qacΔE1 gene. In addition, in 5 isolates of Acinetobacter bumanii, 2 isolates (40%) had the qacE gene, and 4 isolates (80%) had the qacΔE1 gene. CONCLUSIONS: This study shows that the genes which harbored resistance to quaternary ammonium compound antiseptics are widespread among Pseudomonas aeruginosa and Acinetobacter bumanii isolates in burn patients.
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spelling pubmed-40363842014-05-28 Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients Mahzounieh, Mohammadreza Khoshnood, Sheida Ebrahimi, Azizollah Habibian, Saeid Yaghoubian, Maryam Jundishapur J Nat Pharm Prod Research Article BACKGROUND: Quaternary ammonium compounds (QAC), which contain benzalkonium chloride as the most widely used agent, are employed as wound and skin antiseptics, as well as disinfectants in hospitals. The resistance mechanism to disinfectants is usually determine by genes which are related to resistance to quaternary ammonium compounds, namely, qacE, qacΔE1, qacΔE1 that are found in Gram-negative bacteria. OBJECTIVES: The aim of this study was to determine the incidence of antiseptic resistance genes, qacE and qacΔE1, in clinical isolates of Pseudomonas aeruginosa and Acinetobacter bumanii. MATERIALS AND METHODS: In this study, 83 clinical isolates of Pseudomonas aeruginosa, and 5 isolates of Acinetobacter baumannii from burn hospitals in Tehran and Isfahan provinces in 2010-2011, were tested by the PCR method. RESULTS: Out of the 83 clinical isolates of Pseudomonas aeruginosa, 49 isolates (50%) had the qacE gene, and 76 isolates (91.5%) had the qacΔE1 gene. In addition, in 5 isolates of Acinetobacter bumanii, 2 isolates (40%) had the qacE gene, and 4 isolates (80%) had the qacΔE1 gene. CONCLUSIONS: This study shows that the genes which harbored resistance to quaternary ammonium compound antiseptics are widespread among Pseudomonas aeruginosa and Acinetobacter bumanii isolates in burn patients. DOCS 2014-04-03 /pmc/articles/PMC4036384/ /pubmed/24872941 Text en Copyright © 2014, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences; Published by DOCS. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mahzounieh, Mohammadreza
Khoshnood, Sheida
Ebrahimi, Azizollah
Habibian, Saeid
Yaghoubian, Maryam
Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title_full Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title_fullStr Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title_full_unstemmed Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title_short Detection of Antiseptic-Resistance Genes in Pseudomonas and Acinetobacter spp. Isolated From Burn Patients
title_sort detection of antiseptic-resistance genes in pseudomonas and acinetobacter spp. isolated from burn patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036384/
https://www.ncbi.nlm.nih.gov/pubmed/24872941
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