Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (S...

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Autores principales: Cautivo, Karla, Schountz, Tony, Acuña-Retamar, Mariana, Ferrés, Marcela, Torres-Pérez, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036537/
https://www.ncbi.nlm.nih.gov/pubmed/24806874
http://dx.doi.org/10.3390/v6052028
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author Cautivo, Karla
Schountz, Tony
Acuña-Retamar, Mariana
Ferrés, Marcela
Torres-Pérez, Fernando
author_facet Cautivo, Karla
Schountz, Tony
Acuña-Retamar, Mariana
Ferrés, Marcela
Torres-Pérez, Fernando
author_sort Cautivo, Karla
collection PubMed
description We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.
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spelling pubmed-40365372014-05-28 Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals Cautivo, Karla Schountz, Tony Acuña-Retamar, Mariana Ferrés, Marcela Torres-Pérez, Fernando Viruses Short Communication We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species. MDPI 2014-05-06 /pmc/articles/PMC4036537/ /pubmed/24806874 http://dx.doi.org/10.3390/v6052028 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Communication
Cautivo, Karla
Schountz, Tony
Acuña-Retamar, Mariana
Ferrés, Marcela
Torres-Pérez, Fernando
Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title_full Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title_fullStr Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title_full_unstemmed Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title_short Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
title_sort rapid enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036537/
https://www.ncbi.nlm.nih.gov/pubmed/24806874
http://dx.doi.org/10.3390/v6052028
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