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Yeast surface display for protein engineering and characterization

Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries...

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Detalles Bibliográficos
Autores principales: Gai, S Annie, Wittrup, K Dane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038029/
https://www.ncbi.nlm.nih.gov/pubmed/17870469
http://dx.doi.org/10.1016/j.sbi.2007.08.012
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author Gai, S Annie
Wittrup, K Dane
author_facet Gai, S Annie
Wittrup, K Dane
author_sort Gai, S Annie
collection PubMed
description Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein–protein interactions, represents a significant recent advance.
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spelling pubmed-40380292014-05-29 Yeast surface display for protein engineering and characterization Gai, S Annie Wittrup, K Dane Curr Opin Struct Biol Article Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein–protein interactions, represents a significant recent advance. Elsevier Ltd. 2007-08 2007-09-17 /pmc/articles/PMC4038029/ /pubmed/17870469 http://dx.doi.org/10.1016/j.sbi.2007.08.012 Text en Copyright © 2007 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gai, S Annie
Wittrup, K Dane
Yeast surface display for protein engineering and characterization
title Yeast surface display for protein engineering and characterization
title_full Yeast surface display for protein engineering and characterization
title_fullStr Yeast surface display for protein engineering and characterization
title_full_unstemmed Yeast surface display for protein engineering and characterization
title_short Yeast surface display for protein engineering and characterization
title_sort yeast surface display for protein engineering and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038029/
https://www.ncbi.nlm.nih.gov/pubmed/17870469
http://dx.doi.org/10.1016/j.sbi.2007.08.012
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