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The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast

Heterochromatin preferentially assembles at repetitive DNA elements, playing roles in transcriptional silencing, recombination suppression, and chromosome segregation. The RNAi machinery is required for heterochromatin assembly in a diverse range of organisms. In fission yeast, RNA splicing factors...

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Autores principales: Kallgren, Scott P., Andrews, Stuart, Tadeo, Xavier, Hou, Haitong, Moresco, James J., Tu, Patricia G., Yates, John R., Nagy, Peter L., Jia, Songtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038458/
https://www.ncbi.nlm.nih.gov/pubmed/24874881
http://dx.doi.org/10.1371/journal.pgen.1004334
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author Kallgren, Scott P.
Andrews, Stuart
Tadeo, Xavier
Hou, Haitong
Moresco, James J.
Tu, Patricia G.
Yates, John R.
Nagy, Peter L.
Jia, Songtao
author_facet Kallgren, Scott P.
Andrews, Stuart
Tadeo, Xavier
Hou, Haitong
Moresco, James J.
Tu, Patricia G.
Yates, John R.
Nagy, Peter L.
Jia, Songtao
author_sort Kallgren, Scott P.
collection PubMed
description Heterochromatin preferentially assembles at repetitive DNA elements, playing roles in transcriptional silencing, recombination suppression, and chromosome segregation. The RNAi machinery is required for heterochromatin assembly in a diverse range of organisms. In fission yeast, RNA splicing factors are also required for pericentric heterochromatin assembly, and a prevailing model is that splicing factors provide a platform for siRNA generation independently of their splicing activity. Here, by screening the fission yeast deletion library, we discovered four novel splicing factors that are required for pericentric heterochromatin assembly. Sequencing total cellular RNAs from the strongest of these mutants, cwf14Δ, showed intron retention in mRNAs of several RNAi factors. Moreover, introducing cDNA versions of RNAi factors significantly restored pericentric heterochromatin in splicing mutants. We also found that mutations of splicing factors resulted in defective telomeric heterochromatin assembly and mis-splicing the mRNA of shelterin component Tpz1, and that replacement of tpz1(+) with its cDNA partially rescued heterochromatin defects at telomeres in splicing mutants. Thus, proper splicing of RNAi and shelterin factors contributes to heterochromatin assembly at pericentric regions and telomeres.
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spelling pubmed-40384582014-06-05 The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast Kallgren, Scott P. Andrews, Stuart Tadeo, Xavier Hou, Haitong Moresco, James J. Tu, Patricia G. Yates, John R. Nagy, Peter L. Jia, Songtao PLoS Genet Research Article Heterochromatin preferentially assembles at repetitive DNA elements, playing roles in transcriptional silencing, recombination suppression, and chromosome segregation. The RNAi machinery is required for heterochromatin assembly in a diverse range of organisms. In fission yeast, RNA splicing factors are also required for pericentric heterochromatin assembly, and a prevailing model is that splicing factors provide a platform for siRNA generation independently of their splicing activity. Here, by screening the fission yeast deletion library, we discovered four novel splicing factors that are required for pericentric heterochromatin assembly. Sequencing total cellular RNAs from the strongest of these mutants, cwf14Δ, showed intron retention in mRNAs of several RNAi factors. Moreover, introducing cDNA versions of RNAi factors significantly restored pericentric heterochromatin in splicing mutants. We also found that mutations of splicing factors resulted in defective telomeric heterochromatin assembly and mis-splicing the mRNA of shelterin component Tpz1, and that replacement of tpz1(+) with its cDNA partially rescued heterochromatin defects at telomeres in splicing mutants. Thus, proper splicing of RNAi and shelterin factors contributes to heterochromatin assembly at pericentric regions and telomeres. Public Library of Science 2014-05-29 /pmc/articles/PMC4038458/ /pubmed/24874881 http://dx.doi.org/10.1371/journal.pgen.1004334 Text en © 2014 Kallgren et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kallgren, Scott P.
Andrews, Stuart
Tadeo, Xavier
Hou, Haitong
Moresco, James J.
Tu, Patricia G.
Yates, John R.
Nagy, Peter L.
Jia, Songtao
The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title_full The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title_fullStr The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title_full_unstemmed The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title_short The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast
title_sort proper splicing of rnai factors is critical for pericentric heterochromatin assembly in fission yeast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038458/
https://www.ncbi.nlm.nih.gov/pubmed/24874881
http://dx.doi.org/10.1371/journal.pgen.1004334
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