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SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner
The Forkhead transcription factor FOXA2 plays a fundamental role in controlling metabolic homeostasis in the liver during fasting. The precise molecular regulation of FOXA2 in response to nutrients is not fully understood. Here, we studied whether FOXA2 could be controlled at a post-translational le...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038515/ https://www.ncbi.nlm.nih.gov/pubmed/24875183 http://dx.doi.org/10.1371/journal.pone.0098438 |
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author | van Gent, Rogier Di Sanza, Claudio van den Broek, Niels J. F. Fleskens, Veerle Veenstra, Aukje Stout, Gerdine J. Brenkman, Arjan B. |
author_facet | van Gent, Rogier Di Sanza, Claudio van den Broek, Niels J. F. Fleskens, Veerle Veenstra, Aukje Stout, Gerdine J. Brenkman, Arjan B. |
author_sort | van Gent, Rogier |
collection | PubMed |
description | The Forkhead transcription factor FOXA2 plays a fundamental role in controlling metabolic homeostasis in the liver during fasting. The precise molecular regulation of FOXA2 in response to nutrients is not fully understood. Here, we studied whether FOXA2 could be controlled at a post-translational level by acetylation. By means of LC-MS/MS analyses, we identified five acetylated residues in FOXA2. Sirtuin family member SIRT1 was found to interact with and deacetylate FOXA2, the latter process being dependent on the NAD(+)-binding catalytic site of SIRT1. Deacetylation by SIRT1 reduced protein stability of FOXA2 by targeting it towards proteasomal degradation, and inhibited transcription from the FOXA2-driven G6pase and CPT1a promoters. While mutation of the five identified acetylated residues weakly affected protein acetylation and stability, mutation of at least seven additional lysine residues was required to abolish acetylation and reduce protein levels of FOXA2. The importance of acetylation of FOXA2 became apparent upon changes in nutrient levels. The interaction of FOXA2 and SIRT1 was strongly reduced upon nutrient withdrawal in cell culture, while enhanced Foxa2 acetylation levels were observed in murine liver in vivo after starvation for 36 hours. Collectively, this study demonstrates that SIRT1 controls the acetylation level of FOXA2 in a nutrient-dependent manner and in times of nutrient shortage the interaction between SIRT1 and FOXA2 is reduced. As a result, FOXA2 is protected from degradation by enhanced acetylation, hence enabling the FOXA2 transcriptional program to be executed to maintain metabolic homeostasis. |
format | Online Article Text |
id | pubmed-4038515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40385152014-06-05 SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner van Gent, Rogier Di Sanza, Claudio van den Broek, Niels J. F. Fleskens, Veerle Veenstra, Aukje Stout, Gerdine J. Brenkman, Arjan B. PLoS One Research Article The Forkhead transcription factor FOXA2 plays a fundamental role in controlling metabolic homeostasis in the liver during fasting. The precise molecular regulation of FOXA2 in response to nutrients is not fully understood. Here, we studied whether FOXA2 could be controlled at a post-translational level by acetylation. By means of LC-MS/MS analyses, we identified five acetylated residues in FOXA2. Sirtuin family member SIRT1 was found to interact with and deacetylate FOXA2, the latter process being dependent on the NAD(+)-binding catalytic site of SIRT1. Deacetylation by SIRT1 reduced protein stability of FOXA2 by targeting it towards proteasomal degradation, and inhibited transcription from the FOXA2-driven G6pase and CPT1a promoters. While mutation of the five identified acetylated residues weakly affected protein acetylation and stability, mutation of at least seven additional lysine residues was required to abolish acetylation and reduce protein levels of FOXA2. The importance of acetylation of FOXA2 became apparent upon changes in nutrient levels. The interaction of FOXA2 and SIRT1 was strongly reduced upon nutrient withdrawal in cell culture, while enhanced Foxa2 acetylation levels were observed in murine liver in vivo after starvation for 36 hours. Collectively, this study demonstrates that SIRT1 controls the acetylation level of FOXA2 in a nutrient-dependent manner and in times of nutrient shortage the interaction between SIRT1 and FOXA2 is reduced. As a result, FOXA2 is protected from degradation by enhanced acetylation, hence enabling the FOXA2 transcriptional program to be executed to maintain metabolic homeostasis. Public Library of Science 2014-05-29 /pmc/articles/PMC4038515/ /pubmed/24875183 http://dx.doi.org/10.1371/journal.pone.0098438 Text en © 2014 van Gent et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article van Gent, Rogier Di Sanza, Claudio van den Broek, Niels J. F. Fleskens, Veerle Veenstra, Aukje Stout, Gerdine J. Brenkman, Arjan B. SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title | SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title_full | SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title_fullStr | SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title_full_unstemmed | SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title_short | SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner |
title_sort | sirt1 mediates foxa2 breakdown by deacetylation in a nutrient-dependent manner |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4038515/ https://www.ncbi.nlm.nih.gov/pubmed/24875183 http://dx.doi.org/10.1371/journal.pone.0098438 |
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