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Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer

OBJECTIVE: This study is to investigate the role of miR-143 expression in cervical squamous cell carcinoma (SCC). METHODS: The expression level of miR-143 was examined by quantitative real-time PCR. Human papillomavirus (HPV) genotype was detected by HPV genotype detection kit. The expression level...

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Autores principales: Chen, Yanxia, Ma, Cailing, Zhang, Wei, Chen, Zhifang, Ma, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039059/
https://www.ncbi.nlm.nih.gov/pubmed/24774218
http://dx.doi.org/10.1186/1746-1596-9-88
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author Chen, Yanxia
Ma, Cailing
Zhang, Wei
Chen, Zhifang
Ma, Li
author_facet Chen, Yanxia
Ma, Cailing
Zhang, Wei
Chen, Zhifang
Ma, Li
author_sort Chen, Yanxia
collection PubMed
description OBJECTIVE: This study is to investigate the role of miR-143 expression in cervical squamous cell carcinoma (SCC). METHODS: The expression level of miR-143 was examined by quantitative real-time PCR. Human papillomavirus (HPV) genotype was detected by HPV genotype detection kit. The expression level of bcl-2 was detected by immunohistochemistry. RESULTS: The positive rate of HPV was 78% in the patients of cervical SCC. The most prevalent genotype was HPV16, with a positive rate of 42%. The expression level of miR-143 was significantly lower in the cervical SCC tissues than that in the normal cervical tissues (Z = −2.180, P = 0.029). Down-regulated miR-143 expression was associated with tumor size, lymph node metastasis and HPV16 infection in cervical cancer patients. No significant associations were found between the expression levels of miR-143 and age, clinical stage, differentiation or lymph vascular space invasion. And, in cervical SCC patients after treatment with Taxol chemotherapy, the expression level of miR-143 was higher and the positive expression of bcl-2 protein was lower. However, the differences in expression changes of miR-143 and bcl-2 were not statistically significant (miR-143, Z = −0.763, P = 0.446; bcl-2 protein, χ(2) = 2.277, P = 0.131). CONCLUSION: Down-regulated miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical SCC, but miR-143 does not participate in the Taxol sensitivity response. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1401279451112150.
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spelling pubmed-40390592014-05-31 Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer Chen, Yanxia Ma, Cailing Zhang, Wei Chen, Zhifang Ma, Li Diagn Pathol Research OBJECTIVE: This study is to investigate the role of miR-143 expression in cervical squamous cell carcinoma (SCC). METHODS: The expression level of miR-143 was examined by quantitative real-time PCR. Human papillomavirus (HPV) genotype was detected by HPV genotype detection kit. The expression level of bcl-2 was detected by immunohistochemistry. RESULTS: The positive rate of HPV was 78% in the patients of cervical SCC. The most prevalent genotype was HPV16, with a positive rate of 42%. The expression level of miR-143 was significantly lower in the cervical SCC tissues than that in the normal cervical tissues (Z = −2.180, P = 0.029). Down-regulated miR-143 expression was associated with tumor size, lymph node metastasis and HPV16 infection in cervical cancer patients. No significant associations were found between the expression levels of miR-143 and age, clinical stage, differentiation or lymph vascular space invasion. And, in cervical SCC patients after treatment with Taxol chemotherapy, the expression level of miR-143 was higher and the positive expression of bcl-2 protein was lower. However, the differences in expression changes of miR-143 and bcl-2 were not statistically significant (miR-143, Z = −0.763, P = 0.446; bcl-2 protein, χ(2) = 2.277, P = 0.131). CONCLUSION: Down-regulated miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical SCC, but miR-143 does not participate in the Taxol sensitivity response. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1401279451112150. BioMed Central 2014-04-28 /pmc/articles/PMC4039059/ /pubmed/24774218 http://dx.doi.org/10.1186/1746-1596-9-88 Text en Copyright © 2014 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chen, Yanxia
Ma, Cailing
Zhang, Wei
Chen, Zhifang
Ma, Li
Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title_full Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title_fullStr Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title_full_unstemmed Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title_short Down regulation of miR-143 is related with tumor size, lymph node metastasis and HPV16 infection in cervical squamous cancer
title_sort down regulation of mir-143 is related with tumor size, lymph node metastasis and hpv16 infection in cervical squamous cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039059/
https://www.ncbi.nlm.nih.gov/pubmed/24774218
http://dx.doi.org/10.1186/1746-1596-9-88
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