Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor

[Image: see text] We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target an...

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Autores principales: Wang, Liang, Han, Yujing, Zhou, Shuo, Wang, Guihua, Guan, Xiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039345/
https://www.ncbi.nlm.nih.gov/pubmed/24806593
http://dx.doi.org/10.1021/am500749p
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author Wang, Liang
Han, Yujing
Zhou, Shuo
Wang, Guihua
Guan, Xiyun
author_facet Wang, Liang
Han, Yujing
Zhou, Shuo
Wang, Guihua
Guan, Xiyun
author_sort Wang, Liang
collection PubMed
description [Image: see text] We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.
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spelling pubmed-40393452015-05-07 Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor Wang, Liang Han, Yujing Zhou, Shuo Wang, Guihua Guan, Xiyun ACS Appl Mater Interfaces [Image: see text] We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens. American Chemical Society 2014-05-07 2014-05-28 /pmc/articles/PMC4039345/ /pubmed/24806593 http://dx.doi.org/10.1021/am500749p Text en Copyright © 2014 American Chemical Society
spellingShingle Wang, Liang
Han, Yujing
Zhou, Shuo
Wang, Guihua
Guan, Xiyun
Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title_full Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title_fullStr Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title_full_unstemmed Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title_short Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor
title_sort nanopore biosensor for label-free and real-time detection of anthrax lethal factor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039345/
https://www.ncbi.nlm.nih.gov/pubmed/24806593
http://dx.doi.org/10.1021/am500749p
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