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Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and...

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Autores principales: Cheng, Boran, He, Zhaobo, Zhao, Libo, Fang, Yuan, Chen, Yuanyuan, He, Rongxiang, Chen, Fangfang, Song, Haibin, Deng, Yuliang, Zhao, Xingzhong, Xiong, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039424/
https://www.ncbi.nlm.nih.gov/pubmed/24904216
http://dx.doi.org/10.2147/IJN.S61233
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author Cheng, Boran
He, Zhaobo
Zhao, Libo
Fang, Yuan
Chen, Yuanyuan
He, Rongxiang
Chen, Fangfang
Song, Haibin
Deng, Yuliang
Zhao, Xingzhong
Xiong, Bin
author_facet Cheng, Boran
He, Zhaobo
Zhao, Libo
Fang, Yuan
Chen, Yuanyuan
He, Rongxiang
Chen, Fangfang
Song, Haibin
Deng, Yuliang
Zhao, Xingzhong
Xiong, Bin
author_sort Cheng, Boran
collection PubMed
description Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies.
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spelling pubmed-40394242014-06-05 Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture Cheng, Boran He, Zhaobo Zhao, Libo Fang, Yuan Chen, Yuanyuan He, Rongxiang Chen, Fangfang Song, Haibin Deng, Yuliang Zhao, Xingzhong Xiong, Bin Int J Nanomedicine Original Research Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies. Dove Medical Press 2014-05-22 /pmc/articles/PMC4039424/ /pubmed/24904216 http://dx.doi.org/10.2147/IJN.S61233 Text en © Cheng et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Cheng, Boran
He, Zhaobo
Zhao, Libo
Fang, Yuan
Chen, Yuanyuan
He, Rongxiang
Chen, Fangfang
Song, Haibin
Deng, Yuliang
Zhao, Xingzhong
Xiong, Bin
Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_full Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_fullStr Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_full_unstemmed Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_short Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
title_sort transparent, biocompatible nanostructured surfaces for cancer cell capture and culture
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039424/
https://www.ncbi.nlm.nih.gov/pubmed/24904216
http://dx.doi.org/10.2147/IJN.S61233
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