A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique – USER cloning – to rapidly construct mammalian expression vectors of multiple DNA fragments...

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Detalles Bibliográficos
Autores principales: Lund, Anne Mathilde, Kildegaard, Helene Faustrup, Petersen, Maja Borup Kjær, Rank, Julie, Hansen, Bjarne Gram, Andersen, Mikael Rørdam, Mortensen, Uffe Hasbro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039435/
https://www.ncbi.nlm.nih.gov/pubmed/24879460
http://dx.doi.org/10.1371/journal.pone.0096693

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039435/
https://www.ncbi.nlm.nih.gov/pubmed/24879460
http://dx.doi.org/10.1371/journal.pone.0096693

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