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A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique – USER cloning – to rapidly construct mammalian expression vectors of multiple DNA fragments...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039435/ https://www.ncbi.nlm.nih.gov/pubmed/24879460 http://dx.doi.org/10.1371/journal.pone.0096693 |