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Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury

BACKGROUND: Hepatic ischemia-reperfusion (I/R) is a well-studied model of liver injury and has demonstrated a biphasic injury followed by recovery and regeneration. Microparticles (MPs) are a developing field of study and these small membrane bound vesicles have been shown to have effector function...

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Autores principales: Freeman, Christopher M., Quillin, Ralph C., Wilson, Gregory C., Nojima, Hiroyuki, Johnson, Bobby L., Sutton, Jeffrey M., Schuster, Rebecca M., Blanchard, John, Edwards, Michael J., Caldwell, Charles C., Lentsch, Alex B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039439/
https://www.ncbi.nlm.nih.gov/pubmed/24879335
http://dx.doi.org/10.1371/journal.pone.0097945
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author Freeman, Christopher M.
Quillin, Ralph C.
Wilson, Gregory C.
Nojima, Hiroyuki
Johnson, Bobby L.
Sutton, Jeffrey M.
Schuster, Rebecca M.
Blanchard, John
Edwards, Michael J.
Caldwell, Charles C.
Lentsch, Alex B.
author_facet Freeman, Christopher M.
Quillin, Ralph C.
Wilson, Gregory C.
Nojima, Hiroyuki
Johnson, Bobby L.
Sutton, Jeffrey M.
Schuster, Rebecca M.
Blanchard, John
Edwards, Michael J.
Caldwell, Charles C.
Lentsch, Alex B.
author_sort Freeman, Christopher M.
collection PubMed
description BACKGROUND: Hepatic ischemia-reperfusion (I/R) is a well-studied model of liver injury and has demonstrated a biphasic injury followed by recovery and regeneration. Microparticles (MPs) are a developing field of study and these small membrane bound vesicles have been shown to have effector function in other physiologic and pathologic states. This study was designed to quantify the levels of MPs from various cell origins–platelets, neutrophils, and endolethial cells–following hepatic ischemia-reperfusion injury. METHODS: A murine model was used with mice undergoing 90 minutes of partial hepatic ischemia followed by various times of reperfusion. Following reperfusion, plasma samples were taken and MPs of various cell origins were labeled and levels were measured using flow cytometry. Additionally, cell specific MPs were further assessed by Annexin V, which stains for the presence of phosphatidylserine, a cell surface marker linked to apoptosis. Statistical analysis was performed using one-way analysis of variance with subsequent Student-Newman-Keuls test with data presented as the mean and standard error of the mean. RESULTS: MPs from varying sources show an increase in circulating levels following hepatic I/R injury. However, the timing of the appearance of different MP subtypes differs for each cell type. Platelet and neutrophil-derived MP levels demonstrated an acute elevation following injury whereas endothelial-derived MP levels demonstrated a delayed elevation. CONCLUSION: This is the first study to characterize circulating levels of cell-specific MPs after hepatic I/R injury and suggests that MPs derived from platelets and neutrophils serve as markers of inflammatory injury and may be active participants in this process. In contrast, MPs derived from endothelial cells increase after the injury response during the reparative phase and may be important in angiogenesis that occurs in the regenerating liver.
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spelling pubmed-40394392014-06-02 Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury Freeman, Christopher M. Quillin, Ralph C. Wilson, Gregory C. Nojima, Hiroyuki Johnson, Bobby L. Sutton, Jeffrey M. Schuster, Rebecca M. Blanchard, John Edwards, Michael J. Caldwell, Charles C. Lentsch, Alex B. PLoS One Research Article BACKGROUND: Hepatic ischemia-reperfusion (I/R) is a well-studied model of liver injury and has demonstrated a biphasic injury followed by recovery and regeneration. Microparticles (MPs) are a developing field of study and these small membrane bound vesicles have been shown to have effector function in other physiologic and pathologic states. This study was designed to quantify the levels of MPs from various cell origins–platelets, neutrophils, and endolethial cells–following hepatic ischemia-reperfusion injury. METHODS: A murine model was used with mice undergoing 90 minutes of partial hepatic ischemia followed by various times of reperfusion. Following reperfusion, plasma samples were taken and MPs of various cell origins were labeled and levels were measured using flow cytometry. Additionally, cell specific MPs were further assessed by Annexin V, which stains for the presence of phosphatidylserine, a cell surface marker linked to apoptosis. Statistical analysis was performed using one-way analysis of variance with subsequent Student-Newman-Keuls test with data presented as the mean and standard error of the mean. RESULTS: MPs from varying sources show an increase in circulating levels following hepatic I/R injury. However, the timing of the appearance of different MP subtypes differs for each cell type. Platelet and neutrophil-derived MP levels demonstrated an acute elevation following injury whereas endothelial-derived MP levels demonstrated a delayed elevation. CONCLUSION: This is the first study to characterize circulating levels of cell-specific MPs after hepatic I/R injury and suggests that MPs derived from platelets and neutrophils serve as markers of inflammatory injury and may be active participants in this process. In contrast, MPs derived from endothelial cells increase after the injury response during the reparative phase and may be important in angiogenesis that occurs in the regenerating liver. Public Library of Science 2014-05-30 /pmc/articles/PMC4039439/ /pubmed/24879335 http://dx.doi.org/10.1371/journal.pone.0097945 Text en © 2014 Freeman et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Freeman, Christopher M.
Quillin, Ralph C.
Wilson, Gregory C.
Nojima, Hiroyuki
Johnson, Bobby L.
Sutton, Jeffrey M.
Schuster, Rebecca M.
Blanchard, John
Edwards, Michael J.
Caldwell, Charles C.
Lentsch, Alex B.
Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title_full Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title_fullStr Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title_full_unstemmed Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title_short Characterization of Microparticles after Hepatic Ischemia-Reperfusion Injury
title_sort characterization of microparticles after hepatic ischemia-reperfusion injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039439/
https://www.ncbi.nlm.nih.gov/pubmed/24879335
http://dx.doi.org/10.1371/journal.pone.0097945
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