Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

Functional small biomolecules play indispensable roles inside cells. However, sensitive and specific visualization of these molecules in living systems has proven to be highly challenging. Herein, we report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad...

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Detalles Bibliográficos
Autores principales: Wei, Lu, Hu, Fanghao, Shen, Yihui, Chen, Zhixing, Yu, Yong, Lin, Chih-Chun, Wang, Meng C., Min, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040164/
https://www.ncbi.nlm.nih.gov/pubmed/24584195
http://dx.doi.org/10.1038/nmeth.2878
Descripción
Sumario:Functional small biomolecules play indispensable roles inside cells. However, sensitive and specific visualization of these molecules in living systems has proven to be highly challenging. Herein, we report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues, and visualizing de novo synthesis of DNA, RNA, proteomes, phospholipids and triglycerides, respectively, through metabolic incorporation of alkyne-tagged small precursors.

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