Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

Functional small biomolecules play indispensable roles inside cells. However, sensitive and specific visualization of these molecules in living systems has proven to be highly challenging. Herein, we report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad...

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Detalles Bibliográficos
Autores principales: Wei, Lu, Hu, Fanghao, Shen, Yihui, Chen, Zhixing, Yu, Yong, Lin, Chih-Chun, Wang, Meng C., Min, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040164/
https://www.ncbi.nlm.nih.gov/pubmed/24584195
http://dx.doi.org/10.1038/nmeth.2878
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author Wei, Lu
Hu, Fanghao
Shen, Yihui
Chen, Zhixing
Yu, Yong
Lin, Chih-Chun
Wang, Meng C.
Min, Wei
author_facet Wei, Lu
Hu, Fanghao
Shen, Yihui
Chen, Zhixing
Yu, Yong
Lin, Chih-Chun
Wang, Meng C.
Min, Wei
author_sort Wei, Lu
collection PubMed
description Functional small biomolecules play indispensable roles inside cells. However, sensitive and specific visualization of these molecules in living systems has proven to be highly challenging. Herein, we report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues, and visualizing de novo synthesis of DNA, RNA, proteomes, phospholipids and triglycerides, respectively, through metabolic incorporation of alkyne-tagged small precursors.
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spelling pubmed-40401642014-10-01 Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering Wei, Lu Hu, Fanghao Shen, Yihui Chen, Zhixing Yu, Yong Lin, Chih-Chun Wang, Meng C. Min, Wei Nat Methods Article Functional small biomolecules play indispensable roles inside cells. However, sensitive and specific visualization of these molecules in living systems has proven to be highly challenging. Herein, we report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues, and visualizing de novo synthesis of DNA, RNA, proteomes, phospholipids and triglycerides, respectively, through metabolic incorporation of alkyne-tagged small precursors. 2014-03-02 2014-04 /pmc/articles/PMC4040164/ /pubmed/24584195 http://dx.doi.org/10.1038/nmeth.2878 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wei, Lu
Hu, Fanghao
Shen, Yihui
Chen, Zhixing
Yu, Yong
Lin, Chih-Chun
Wang, Meng C.
Min, Wei
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title_full Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title_fullStr Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title_full_unstemmed Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title_short Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
title_sort live-cell imaging of alkyne-tagged small biomolecules by stimulated raman scattering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040164/
https://www.ncbi.nlm.nih.gov/pubmed/24584195
http://dx.doi.org/10.1038/nmeth.2878
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