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Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing
Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh benthic trophic structure. Indeed, salt marsh...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040508/ https://www.ncbi.nlm.nih.gov/pubmed/24917856 http://dx.doi.org/10.3389/fmicb.2014.00263 |
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author | Darjany, Lindsay E. Whitcraft, Christine R. Dillon, Jesse G. |
author_facet | Darjany, Lindsay E. Whitcraft, Christine R. Dillon, Jesse G. |
author_sort | Darjany, Lindsay E. |
collection | PubMed |
description | Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh benthic trophic structure. Indeed, salt marsh sediment bacteria remain largely in a black box in terms of their diversity and functional roles within salt marsh benthic food web pathways. We used DNA stable isotope probing (SIP) utilizing (13)C-labeled lignocellulose as a proxy to evaluate the fate of macrophyte-derived carbon in benthic salt marsh bacterial communities. Overall, 146 bacterial species were detected using SIP, of which only 12 lineages were shared between enriched and non-enriched communities. Abundant groups from the (13)C-labeled community included Desulfosarcina, Spirochaeta, and Kangiella. This study is the first to use heavy-labeled lignocellulose to identify bacteria responsible for macrophyte carbon utilization in salt marsh sediments and will allow future studies to target specific lineages to elucidate their role in salt marsh carbon cycling and ultimately aid our understanding of the potential of salt marshes to store carbon. |
format | Online Article Text |
id | pubmed-4040508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-40405082014-06-10 Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing Darjany, Lindsay E. Whitcraft, Christine R. Dillon, Jesse G. Front Microbiol Microbiology Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh benthic trophic structure. Indeed, salt marsh sediment bacteria remain largely in a black box in terms of their diversity and functional roles within salt marsh benthic food web pathways. We used DNA stable isotope probing (SIP) utilizing (13)C-labeled lignocellulose as a proxy to evaluate the fate of macrophyte-derived carbon in benthic salt marsh bacterial communities. Overall, 146 bacterial species were detected using SIP, of which only 12 lineages were shared between enriched and non-enriched communities. Abundant groups from the (13)C-labeled community included Desulfosarcina, Spirochaeta, and Kangiella. This study is the first to use heavy-labeled lignocellulose to identify bacteria responsible for macrophyte carbon utilization in salt marsh sediments and will allow future studies to target specific lineages to elucidate their role in salt marsh carbon cycling and ultimately aid our understanding of the potential of salt marshes to store carbon. Frontiers Media S.A. 2014-06-02 /pmc/articles/PMC4040508/ /pubmed/24917856 http://dx.doi.org/10.3389/fmicb.2014.00263 Text en Copyright © 2014 Darjany, Whitcraft and Dillon. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Darjany, Lindsay E. Whitcraft, Christine R. Dillon, Jesse G. Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title | Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title_full | Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title_fullStr | Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title_full_unstemmed | Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title_short | Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing |
title_sort | lignocellulose-responsive bacteria in a southern california salt marsh identified by stable isotope probing |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040508/ https://www.ncbi.nlm.nih.gov/pubmed/24917856 http://dx.doi.org/10.3389/fmicb.2014.00263 |
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