DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2

We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yano...

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Autores principales: Hayashi, Hiroe, Kurusu, Yasurou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041236/
https://www.ncbi.nlm.nih.gov/pubmed/24441514
http://dx.doi.org/10.1264/jsme2.ME13135
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author Hayashi, Hiroe
Kurusu, Yasurou
author_facet Hayashi, Hiroe
Kurusu, Yasurou
author_sort Hayashi, Hiroe
collection PubMed
description We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli.
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spelling pubmed-40412362014-07-24 DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2 Hayashi, Hiroe Kurusu, Yasurou Microbes Environ Short Communication We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number small plasmid pYAN-2 (4,687 bp) from Sphingobium yanoikuyae. It does not encode any significant protein sequence and has one direct repeat for possible secondary structures. Moreover, a 70 bp fragment, upstream of the above sequence, completely stabilized the unstable pSC101 plasmid in E. coli. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2014-03 2014-01-17 /pmc/articles/PMC4041236/ /pubmed/24441514 http://dx.doi.org/10.1264/jsme2.ME13135 Text en Copyright © 2014 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Hayashi, Hiroe
Kurusu, Yasurou
DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title_full DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title_fullStr DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title_full_unstemmed DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title_short DNA Regions Responsible for Maintenance of Shingobium Plasmid pYAN-2
title_sort dna regions responsible for maintenance of shingobium plasmid pyan-2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041236/
https://www.ncbi.nlm.nih.gov/pubmed/24441514
http://dx.doi.org/10.1264/jsme2.ME13135
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