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Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats
BACKGROUND: Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl(4))-induced oxidative stress and testes injury in adult Wistar rat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041339/ https://www.ncbi.nlm.nih.gov/pubmed/24884677 http://dx.doi.org/10.1186/1472-6882-14-164 |
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author | Al-Olayan, Ebtesam M El-Khadragy, Manal F Metwally, Dina M Abdel Moneim, Ahmed E |
author_facet | Al-Olayan, Ebtesam M El-Khadragy, Manal F Metwally, Dina M Abdel Moneim, Ahmed E |
author_sort | Al-Olayan, Ebtesam M |
collection | PubMed |
description | BACKGROUND: Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl(4))-induced oxidative stress and testes injury in adult Wistar rats. METHODS: Twenty eight Wistar albino male rats were divided equally into 4 groups for the assessment of protective potential of pomegranate juice. Rats of group I (control) received only vehicles and had free access to food and water. Rats of groups II and IV were treated with CCl(4) (2 ml/kg bwt) via the intraperitoneal route once a week for ten weeks. The pomegranate juice was supplemented via drinking water 2 weeks before and concurrent with CCl(4) treatment to group IV. Group III was supplemented with pomegranate juice for twelve weeks. The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl(4)-induced reproductive toxicity in rats. RESULTS: Pomegranate juice showed significant elevation in testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) those depleted by the injection of CCl(4). Activity levels of endogenous testesticular antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and glutathione (GSH) contents were increased while lipid peroxidation (LPO) and nitric oxide (NO) were decreased with pomegranate juice. Moreover, degeneration of germ and Leydig cells along with deformities in spermatogenesis induced after CCl(4) injections were restored with the treatment of pomegranate juice. CONCLUSION: The results clearly demonstrated that pomegranate juice augments the antioxidant defense mechanism against carbon tetrachloride-induced reproductive toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases. |
format | Online Article Text |
id | pubmed-4041339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40413392014-06-03 Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats Al-Olayan, Ebtesam M El-Khadragy, Manal F Metwally, Dina M Abdel Moneim, Ahmed E BMC Complement Altern Med Research Article BACKGROUND: Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl(4))-induced oxidative stress and testes injury in adult Wistar rats. METHODS: Twenty eight Wistar albino male rats were divided equally into 4 groups for the assessment of protective potential of pomegranate juice. Rats of group I (control) received only vehicles and had free access to food and water. Rats of groups II and IV were treated with CCl(4) (2 ml/kg bwt) via the intraperitoneal route once a week for ten weeks. The pomegranate juice was supplemented via drinking water 2 weeks before and concurrent with CCl(4) treatment to group IV. Group III was supplemented with pomegranate juice for twelve weeks. The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl(4)-induced reproductive toxicity in rats. RESULTS: Pomegranate juice showed significant elevation in testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) those depleted by the injection of CCl(4). Activity levels of endogenous testesticular antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and glutathione (GSH) contents were increased while lipid peroxidation (LPO) and nitric oxide (NO) were decreased with pomegranate juice. Moreover, degeneration of germ and Leydig cells along with deformities in spermatogenesis induced after CCl(4) injections were restored with the treatment of pomegranate juice. CONCLUSION: The results clearly demonstrated that pomegranate juice augments the antioxidant defense mechanism against carbon tetrachloride-induced reproductive toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases. BioMed Central 2014-05-22 /pmc/articles/PMC4041339/ /pubmed/24884677 http://dx.doi.org/10.1186/1472-6882-14-164 Text en Copyright © 2014 Al-Olayan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Article Al-Olayan, Ebtesam M El-Khadragy, Manal F Metwally, Dina M Abdel Moneim, Ahmed E Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title | Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title_full | Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title_fullStr | Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title_full_unstemmed | Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title_short | Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
title_sort | protective effects of pomegranate (punica granatum) juice on testes against carbon tetrachloride intoxication in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041339/ https://www.ncbi.nlm.nih.gov/pubmed/24884677 http://dx.doi.org/10.1186/1472-6882-14-164 |
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