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Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices

Mapping transcription factor (TF) binding sites along a DNA backbone is crucial in understanding the regulatory circuits that control cellular processes. Here, we deployed a method adopting bioconjugation, nanofluidic confinement and fluorescence single molecule imaging for direct mapping of TF (RNA...

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Detalles Bibliográficos
Autores principales:	Sriram, K. K., Yeh, Jia-Wei, Lin, Yii-Lih, Chang, Yi-Ren, Chou, Chia-Fu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041428/ https://www.ncbi.nlm.nih.gov/pubmed/24753422 http://dx.doi.org/10.1093/nar/gku254

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