Cargando…

Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics

The Staphylococcus aureus genome contains three toxin–antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zorzini, Valentina, Buts, Lieven, Sleutel, Mike, Garcia-Pino, Abel, Talavera, Ariel, Haesaerts, Sarah, Greve, Henri De, Cheung, Ambrose, van Nuland, Nico A. J., Loris, Remy
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041440/ https://www.ncbi.nlm.nih.gov/pubmed/24748664 http://dx.doi.org/10.1093/nar/gku266

Ejemplares similares

Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
por: Zorzini, Valentina, et al.
Publicado: (2015)

Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a β-strand sliding mechanism
por: Hadži, San, et al.
Publicado: (2017)

SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization
por: Fislage, Marcus, et al.
Publicado: (2014)

A nanobody modulates the p53 transcriptional program without perturbing its functional architecture
por: Bethuyne, Jonas, et al.
Publicado: (2014)

The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu
por: Castro-Roa, Daniel, et al.
Publicado: (2013)

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA
por: Garcia-Rodriguez, Gabriela, et al.
Publicado: (2021)

The Maz’Ya anniversary collection
por: Rossmann, Jürgen, et al.
Publicado: (1999)

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT
por: Talavera, Ariel, et al.
Publicado: (2019)

Genome-wide prediction of minor-groove electrostatic potential enables biophysical modeling of protein–DNA binding
por: Chiu, Tsu-Pei, et al.
Publicado: (2017)

Biophysical basis for convergent evolution of two veil-forming microbes
por: Petroff, Alexander P., et al.
Publicado: (2015)

Biophysical studies of DNA modified with conformationally constrained nucleotides: comparison of 2′-exo (north) and 3′-exo (south) ‘locked’ templates
por: Maderia, Melissa, et al.
Publicado: (2007)

Solution-state NMR structure and biophysical characterization of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis
por: Buchko, Garry W., et al.
Publicado: (2011)

miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif
por: Goldie, Belinda J., et al.
Publicado: (2017)

Around the Research of Vladimir Maz'ya I
por: Laptev, Ari
Publicado: (2010)

Around the Research of Vladimir Maz'ya III
por: Laptev, Ari
Publicado: (2010)

Around the Research of Vladimir Maz'ya II
por: Laptev, Ari, et al.
Publicado: (2010)

Toward Classification of BRCA1 Missense Variants Using a Biophysical Approach
por: Rowling, Pamela J. E., et al.
Publicado: (2010)

Deriving the ultrastructure of α-crustacyanin using lower-resolution structural and biophysical methods
por: Rhys, Natasha H., et al.
Publicado: (2011)

Structural, Biochemical, and Biophysical Characterization of Idelalisib Binding to Phosphoinositide 3-Kinase δ
por: Somoza, John R., et al.
Publicado: (2015)

Biophysical analysis of Pseudomonas-phage PaP3 small terminase suggests a mechanism for sequence-specific DNA-binding by lateral interdigitation
por: Niazi, Marzia, et al.
Publicado: (2020)

Phosphorylation decelerates conformational dynamics in bacterial translation elongation factors
por: Talavera, Ariel, et al.
Publicado: (2018)

Simple biophysics underpins collective conformations of the intrinsically disordered proteins of the Nuclear Pore Complex
por: Vovk, Andrei, et al.
Publicado: (2016)

The transcription factor Maz is essential for normal eye development
por: Medina-Martinez, Olga, et al.
Publicado: (2020)

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum
por: Vuchelen, Anneleen, et al.
Publicado: (2013)

The Toxin-Antitoxin MazEF Drives Staphylococcus aureus Biofilm Formation, Antibiotic Tolerance, and Chronic Infection
por: Ma, Dongzhu, et al.
Publicado: (2019)

Stress-Induced MazF-Mediated Proteins in Escherichia coli
por: Nigam, Akanksha, et al.
Publicado: (2019)

Crystal Structure and Biophysical Properties of Bacillus subtilis BdbD: AN OXIDIZING THIOL:DISULFIDE OXIDOREDUCTASE CONTAINING A NOVEL METAL SITE
por: Crow, Allister, et al.
Publicado: (2009)

A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli
por: Vandierendonck, Jessie, et al.
Publicado: (2023)

Two Programmed Cell Death Systems in Escherichia coli: An Apoptotic-Like Death Is Inhibited by the mazEF-Mediated Death Pathway
por: Erental, Ariel, et al.
Publicado: (2012)

Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations
por: Nikolic, Nela, et al.
Publicado: (2018)

A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
por: Earl, Christopher, et al.
Publicado: (2018)

Customisable IT tool for on-field assessments to support disaster management
por: Grimaz, Stefano, et al.
Publicado: (2023)

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase
por: Cole, Ambrose R., et al.
Publicado: (2013)

Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
por: Myatt, Daniel P, et al.
Publicado: (2023)

Evidence for the Intense Exchange of MazG in Marine Cyanophages by Horizontal Gene Transfer
por: Bryan, Michael J., et al.
Publicado: (2008)

Analysis, partial differential equations and applications: the Vladimir Maz’Ya anniversary volume
por: Cialdea, Alberto, et al.
Publicado: (2009)

tRNA is a new target for cleavage by a MazF toxin
por: Schifano, Jason M., et al.
Publicado: (2016)

RNA Futile Cycling in Model Persisters Derived from MazF Accumulation
por: Mok, Wendy W. K., et al.
Publicado: (2015)

Concerns about “Stress-Induced MazF-Mediated Proteins in Escherichia coli”
por: Wade, Joseph T., et al.
Publicado: (2019)

Reanalysis of Proteomics Results Fails To Detect MazF-Mediated Stress Proteins
por: Kaldalu, Niilo, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_e4rbqh7sa58r16kgbrk2aete8k