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HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment

Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the cho...

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Detalles Bibliográficos
Autores principales: Taniguchi, Ichiro, Mabuchi, Naoto, Ohno, Mutsuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/
https://www.ncbi.nlm.nih.gov/pubmed/24753416
http://dx.doi.org/10.1093/nar/gku304
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author Taniguchi, Ichiro
Mabuchi, Naoto
Ohno, Mutsuhito
author_facet Taniguchi, Ichiro
Mabuchi, Naoto
Ohno, Mutsuhito
author_sort Taniguchi, Ichiro
collection PubMed
description Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the choice is regulated. This issue is highlighted when the human immunodeficiency virus type 1 (HIV-1) Rev protein induces the export of singly spliced and unspliced HIV-1 transcripts. How these RNAs are exported is not well understood because such transcripts should have the possibility of utilizing CRM1-dependent export via Rev or cellular TAP/NXF1-dependent export via the transcription/export (TREX) complex, or both. Here we found that Rev suppressed TAP/NXF1-dependent export of model RNA substrates that recapitulated viral transcripts. In this effect, Rev interacted with the cap-binding complex and inhibited the recruitment of the TREX complex. Thus, Rev controls the identity of the factor occupying the cap-proximal region that determines the RNA export pathway. This ribonucleoprotein remodeling activity of Rev may favor viral gene expression.
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spelling pubmed-40414682014-06-11 HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment Taniguchi, Ichiro Mabuchi, Naoto Ohno, Mutsuhito Nucleic Acids Res RNA Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the choice is regulated. This issue is highlighted when the human immunodeficiency virus type 1 (HIV-1) Rev protein induces the export of singly spliced and unspliced HIV-1 transcripts. How these RNAs are exported is not well understood because such transcripts should have the possibility of utilizing CRM1-dependent export via Rev or cellular TAP/NXF1-dependent export via the transcription/export (TREX) complex, or both. Here we found that Rev suppressed TAP/NXF1-dependent export of model RNA substrates that recapitulated viral transcripts. In this effect, Rev interacted with the cap-binding complex and inhibited the recruitment of the TREX complex. Thus, Rev controls the identity of the factor occupying the cap-proximal region that determines the RNA export pathway. This ribonucleoprotein remodeling activity of Rev may favor viral gene expression. Oxford University Press 2014-06-01 2014-04-20 /pmc/articles/PMC4041468/ /pubmed/24753416 http://dx.doi.org/10.1093/nar/gku304 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited
spellingShingle RNA
Taniguchi, Ichiro
Mabuchi, Naoto
Ohno, Mutsuhito
HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title_full HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title_fullStr HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title_full_unstemmed HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title_short HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment
title_sort hiv-1 rev protein specifies the viral rna export pathway by suppressing tap/nxf1 recruitment
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/
https://www.ncbi.nlm.nih.gov/pubmed/24753416
http://dx.doi.org/10.1093/nar/gku304
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