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RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters
BACKGROUND: Glandular trichomes are production and storage organs of specialized metabolites such as terpenes, which play a role in the plant’s defense system. The present study aimed to shed light on the regulation of terpene biosynthesis in Solanum lycopersicum trichomes by identification of trans...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041997/ https://www.ncbi.nlm.nih.gov/pubmed/24884371 http://dx.doi.org/10.1186/1471-2164-15-402 |
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author | Spyropoulou, Eleni A Haring, Michel A Schuurink, Robert C |
author_facet | Spyropoulou, Eleni A Haring, Michel A Schuurink, Robert C |
author_sort | Spyropoulou, Eleni A |
collection | PubMed |
description | BACKGROUND: Glandular trichomes are production and storage organs of specialized metabolites such as terpenes, which play a role in the plant’s defense system. The present study aimed to shed light on the regulation of terpene biosynthesis in Solanum lycopersicum trichomes by identification of transcription factors (TFs) that control the expression of terpene synthases. RESULTS: A trichome transcriptome database was created with a total of 27,195 contigs that contained 743 annotated TFs. Furthermore a quantitative expression database was obtained of jasmonic acid-treated trichomes. Sixteen candidate TFs were selected for further analysis. One TF of the MYC bHLH class and one of the WRKY class were able to transiently transactivate S. lycopersicum terpene synthase promoters in Nicotiana benthamiana leaves. Strikingly, SlMYC1 was shown to act synergistically with a previously identified zinc finger-like TF, Expression of Terpenoids 1 (SlEOT1) in transactivating the SlTPS5 promoter. CONCLUSIONS: High-throughput sequencing of tomato stem trichomes led to the discovery of two transcription factors that activated several terpene synthase promoters. Our results identified new elements of the transcriptional regulation of tomato terpene biosynthesis in trichomes, a largely unexplored field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-402) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4041997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40419972014-06-06 RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters Spyropoulou, Eleni A Haring, Michel A Schuurink, Robert C BMC Genomics Research Article BACKGROUND: Glandular trichomes are production and storage organs of specialized metabolites such as terpenes, which play a role in the plant’s defense system. The present study aimed to shed light on the regulation of terpene biosynthesis in Solanum lycopersicum trichomes by identification of transcription factors (TFs) that control the expression of terpene synthases. RESULTS: A trichome transcriptome database was created with a total of 27,195 contigs that contained 743 annotated TFs. Furthermore a quantitative expression database was obtained of jasmonic acid-treated trichomes. Sixteen candidate TFs were selected for further analysis. One TF of the MYC bHLH class and one of the WRKY class were able to transiently transactivate S. lycopersicum terpene synthase promoters in Nicotiana benthamiana leaves. Strikingly, SlMYC1 was shown to act synergistically with a previously identified zinc finger-like TF, Expression of Terpenoids 1 (SlEOT1) in transactivating the SlTPS5 promoter. CONCLUSIONS: High-throughput sequencing of tomato stem trichomes led to the discovery of two transcription factors that activated several terpene synthase promoters. Our results identified new elements of the transcriptional regulation of tomato terpene biosynthesis in trichomes, a largely unexplored field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-402) contains supplementary material, which is available to authorized users. BioMed Central 2014-05-27 /pmc/articles/PMC4041997/ /pubmed/24884371 http://dx.doi.org/10.1186/1471-2164-15-402 Text en © Spyropoulou et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Spyropoulou, Eleni A Haring, Michel A Schuurink, Robert C RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title | RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title_full | RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title_fullStr | RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title_full_unstemmed | RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title_short | RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
title_sort | rna sequencing on solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041997/ https://www.ncbi.nlm.nih.gov/pubmed/24884371 http://dx.doi.org/10.1186/1471-2164-15-402 |
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