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Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons

Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. In the present study, we investigated reorganization of the cytoskeleton in neurons infected with two EHV-1 strains: Jan-E (wild-type strain) and Rac-H (attenuated strain). The studies were p...

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Autores principales: Słońska, A., Cymerys, J., Godlewski, M. M., Dzieciątkowski, T., Tucholska, A., Chmielewska, A., Golke, A., Bańbura, M. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042010/
https://www.ncbi.nlm.nih.gov/pubmed/24352436
http://dx.doi.org/10.1007/s00705-013-1949-3
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author Słońska, A.
Cymerys, J.
Godlewski, M. M.
Dzieciątkowski, T.
Tucholska, A.
Chmielewska, A.
Golke, A.
Bańbura, M. W.
author_facet Słońska, A.
Cymerys, J.
Godlewski, M. M.
Dzieciątkowski, T.
Tucholska, A.
Chmielewska, A.
Golke, A.
Bańbura, M. W.
author_sort Słońska, A.
collection PubMed
description Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. In the present study, we investigated reorganization of the cytoskeleton in neurons infected with two EHV-1 strains: Jan-E (wild-type strain) and Rac-H (attenuated strain). The studies were performed on primary murine neurons, which are an excellent model for studying neurotropism and neurovirulence of EHV-1. We have demonstrated for the first time that EHV-1 infection causes rearrangements in the actin network of neurons that are dependent on the virus strain and its adaptation to cell culture in vitro. Immunofluorescent labeling and confocal microscopy revealed the formation of long, thin projections in neurons infected with the Jan-E strain, which was probably associated with enhanced intracellular spread of the virus. The EHV-1 Rac-H strain caused disruption of the microfilaments system and general depolymerization of actin, but treatment of neurons with cytochalasin D or latrunculin A resulted in limitation of viral replication. It can therefore be assumed that actin filaments are required only at the early stages of infection. Our results allow us to suggest that the actin cytoskeleton participates in EHV-1 infection of primary murine neurons but is not essential, and that other components of the cytoskeleton and/or cellular mechanisms may be also involved during EHV-1 infection.
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spelling pubmed-40420102014-06-18 Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons Słońska, A. Cymerys, J. Godlewski, M. M. Dzieciątkowski, T. Tucholska, A. Chmielewska, A. Golke, A. Bańbura, M. W. Arch Virol Original Article Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. In the present study, we investigated reorganization of the cytoskeleton in neurons infected with two EHV-1 strains: Jan-E (wild-type strain) and Rac-H (attenuated strain). The studies were performed on primary murine neurons, which are an excellent model for studying neurotropism and neurovirulence of EHV-1. We have demonstrated for the first time that EHV-1 infection causes rearrangements in the actin network of neurons that are dependent on the virus strain and its adaptation to cell culture in vitro. Immunofluorescent labeling and confocal microscopy revealed the formation of long, thin projections in neurons infected with the Jan-E strain, which was probably associated with enhanced intracellular spread of the virus. The EHV-1 Rac-H strain caused disruption of the microfilaments system and general depolymerization of actin, but treatment of neurons with cytochalasin D or latrunculin A resulted in limitation of viral replication. It can therefore be assumed that actin filaments are required only at the early stages of infection. Our results allow us to suggest that the actin cytoskeleton participates in EHV-1 infection of primary murine neurons but is not essential, and that other components of the cytoskeleton and/or cellular mechanisms may be also involved during EHV-1 infection. Springer Vienna 2013-12-19 2014 /pmc/articles/PMC4042010/ /pubmed/24352436 http://dx.doi.org/10.1007/s00705-013-1949-3 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Słońska, A.
Cymerys, J.
Godlewski, M. M.
Dzieciątkowski, T.
Tucholska, A.
Chmielewska, A.
Golke, A.
Bańbura, M. W.
Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title_full Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title_fullStr Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title_full_unstemmed Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title_short Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
title_sort equine herpesvirus type 1 (ehv-1)-induced rearrangements of actin filaments in productively infected primary murine neurons
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042010/
https://www.ncbi.nlm.nih.gov/pubmed/24352436
http://dx.doi.org/10.1007/s00705-013-1949-3
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