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Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel)
Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042574/ https://www.ncbi.nlm.nih.gov/pubmed/24787269 http://dx.doi.org/10.3390/nu6051823 |
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author | Stasiuk, Maria Janiszewska, Alicja Kozubek, Arkadiusz |
author_facet | Stasiuk, Maria Janiszewska, Alicja Kozubek, Arkadiusz |
author_sort | Stasiuk, Maria |
collection | PubMed |
description | Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemical structure of the phenolic lipids that were available for interaction with the enzyme. All of the tested compounds reduced the activity of acetylcholinesterase. The degree of inhibition varied, showing a correlation with changes in the conformation of the enzyme tested by the intrinsic fluorescence of the Trp residues of the protein. |
format | Online Article Text |
id | pubmed-4042574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-40425742014-06-04 Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) Stasiuk, Maria Janiszewska, Alicja Kozubek, Arkadiusz Nutrients Article Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemical structure of the phenolic lipids that were available for interaction with the enzyme. All of the tested compounds reduced the activity of acetylcholinesterase. The degree of inhibition varied, showing a correlation with changes in the conformation of the enzyme tested by the intrinsic fluorescence of the Trp residues of the protein. MDPI 2014-04-30 /pmc/articles/PMC4042574/ /pubmed/24787269 http://dx.doi.org/10.3390/nu6051823 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Stasiuk, Maria Janiszewska, Alicja Kozubek, Arkadiusz Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title | Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title_full | Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title_fullStr | Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title_full_unstemmed | Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title_short | Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel) |
title_sort | phenolic lipids affect the activity and conformation of acetylcholinesterase from electrophorus electricus (electric eel) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042574/ https://www.ncbi.nlm.nih.gov/pubmed/24787269 http://dx.doi.org/10.3390/nu6051823 |
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