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Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity

An antibacterial substance from Streptococcus sanguinis (S. sanguinis) is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from S. sanguinis....

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Autores principales: MA, SHENGLI, LI, HUI, YAN, CHUANG, WANG, DAN, LI, HAIQING, XIA, XUE, DONG, XUE, ZHAO, YINGNAN, SUN, TINGTING, HU, PENGFEI, GUAN, WEIJUN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043591/
https://www.ncbi.nlm.nih.gov/pubmed/24926331
http://dx.doi.org/10.3892/etm.2014.1618
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author MA, SHENGLI
LI, HUI
YAN, CHUANG
WANG, DAN
LI, HAIQING
XIA, XUE
DONG, XUE
ZHAO, YINGNAN
SUN, TINGTING
HU, PENGFEI
GUAN, WEIJUN
author_facet MA, SHENGLI
LI, HUI
YAN, CHUANG
WANG, DAN
LI, HAIQING
XIA, XUE
DONG, XUE
ZHAO, YINGNAN
SUN, TINGTING
HU, PENGFEI
GUAN, WEIJUN
author_sort MA, SHENGLI
collection PubMed
description An antibacterial substance from Streptococcus sanguinis (S. sanguinis) is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from S. sanguinis. The antagonistic effect of the protein extracts on Prevotella intermedia (P. intermedia) and Porphyromonas gingivalis (P. gingivalis) was detected by a well-plate technique, and the effects of the protein extracts on biofilms formed by these bacteria were evaluated by confocal laser scanning microscopy. The antagonistic effect of the protein extracts on pathogenic fungi was investigated using Candida albicans (C. albicans) and Candida tropicalis (C. tropicalis). The growth curves of C. albicans and C. tropicalis were determined from ultraviolet absorption measurements, their morphological changes following treatment were observed by optical microscopy and scanning electron microscopy, and the effects of the protein extracts on the thickness of their biofilms and the distribution of dead/live bacteria within the biofilms were detected by confocal laser scanning microscopy. The results showed significant inhibitory effects of the intracellular proteins extracted from S. sanguinis on pathogenic bacteria (P. intermedia and P. gingivalis), fungi (C. albicans and C. tropicalis) and the biofilms formed by them. Furthermore, the growth curves and morphology of C. albicans and C. tropicalis were altered following treatment with the intracellular proteins, resulting in disc-like depressions in the surfaces of the fungal spores and mycelia. By contrast, the exocrine proteins demonstrated no significant inhibitory effect on the pathogenic bacteria, fungi and the biofilms formed by them. Thus, it may be concluded that intracellular proteins of S. sanguinis have antibacterial activity and exert an antagonistic effect on certain pathogenic bacteria and fungi of the oral cavity.
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spelling pubmed-40435912014-06-12 Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity MA, SHENGLI LI, HUI YAN, CHUANG WANG, DAN LI, HAIQING XIA, XUE DONG, XUE ZHAO, YINGNAN SUN, TINGTING HU, PENGFEI GUAN, WEIJUN Exp Ther Med Articles An antibacterial substance from Streptococcus sanguinis (S. sanguinis) is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from S. sanguinis. The antagonistic effect of the protein extracts on Prevotella intermedia (P. intermedia) and Porphyromonas gingivalis (P. gingivalis) was detected by a well-plate technique, and the effects of the protein extracts on biofilms formed by these bacteria were evaluated by confocal laser scanning microscopy. The antagonistic effect of the protein extracts on pathogenic fungi was investigated using Candida albicans (C. albicans) and Candida tropicalis (C. tropicalis). The growth curves of C. albicans and C. tropicalis were determined from ultraviolet absorption measurements, their morphological changes following treatment were observed by optical microscopy and scanning electron microscopy, and the effects of the protein extracts on the thickness of their biofilms and the distribution of dead/live bacteria within the biofilms were detected by confocal laser scanning microscopy. The results showed significant inhibitory effects of the intracellular proteins extracted from S. sanguinis on pathogenic bacteria (P. intermedia and P. gingivalis), fungi (C. albicans and C. tropicalis) and the biofilms formed by them. Furthermore, the growth curves and morphology of C. albicans and C. tropicalis were altered following treatment with the intracellular proteins, resulting in disc-like depressions in the surfaces of the fungal spores and mycelia. By contrast, the exocrine proteins demonstrated no significant inhibitory effect on the pathogenic bacteria, fungi and the biofilms formed by them. Thus, it may be concluded that intracellular proteins of S. sanguinis have antibacterial activity and exert an antagonistic effect on certain pathogenic bacteria and fungi of the oral cavity. D.A. Spandidos 2014-06 2014-03-12 /pmc/articles/PMC4043591/ /pubmed/24926331 http://dx.doi.org/10.3892/etm.2014.1618 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
MA, SHENGLI
LI, HUI
YAN, CHUANG
WANG, DAN
LI, HAIQING
XIA, XUE
DONG, XUE
ZHAO, YINGNAN
SUN, TINGTING
HU, PENGFEI
GUAN, WEIJUN
Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title_full Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title_fullStr Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title_full_unstemmed Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title_short Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
title_sort antagonistic effect of protein extracts from streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043591/
https://www.ncbi.nlm.nih.gov/pubmed/24926331
http://dx.doi.org/10.3892/etm.2014.1618
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