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Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model

The aim of the present study was to investigate the effect of erythropoietin (EPO) loading chitosan-tripolyphosphate (CS-TPP) nanoparticles on an immunoglobulin A nephropathy (IgAN) rat model. CS-TPP nanoparticles were produced from CS and TPP and EPO was loaded by mixing with the nanoparticles. The...

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Autores principales: ZHANG, XIAOLI, WU, YIN, SUN, KUN, TAN, JING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043601/
https://www.ncbi.nlm.nih.gov/pubmed/24926362
http://dx.doi.org/10.3892/etm.2014.1643
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author ZHANG, XIAOLI
WU, YIN
SUN, KUN
TAN, JING
author_facet ZHANG, XIAOLI
WU, YIN
SUN, KUN
TAN, JING
author_sort ZHANG, XIAOLI
collection PubMed
description The aim of the present study was to investigate the effect of erythropoietin (EPO) loading chitosan-tripolyphosphate (CS-TPP) nanoparticles on an immunoglobulin A nephropathy (IgAN) rat model. CS-TPP nanoparticles were produced from CS and TPP and EPO was loaded by mixing with the nanoparticles. The IgAN rat models were randomly divided into three groups: the CS-TPP-EPO group, CS-TPP group and EPO group. Hemoglobin (Hb), blood urea nitrogen (BUN) and creatinine (Cr) levels were measured in each group using a Biochemical Analyzer (Hitachi, Tokyo, Japan). The average size of nanoparticles was 485±12 nm and the encapsulation efficiency of EPO was 78.45%. The EPO release curve in CS-TPP-EPO nanoparticles exhibited a biphasic distribution in vitro. The levels of BUN and Cr in the CS-TPP-EPO group were significantly lower compared with the control group (P<0.05); however, the level of Hb in the CS-TPP-EPO group was higher compared with the other groups (P<0.05). The changes in Hb, BUN and Cr in the CS-TPP-EPO group were maintained for less than one week following the end of the treatment with CS-TPP-EPO nanoparticles. In conclusion, the CS-TPP-EPO nanoparticles had a lower toxicity compared with EPO and CS-TPP treatment. Furthermore, CS-TPP-EPO may improve the therapeutic effect in the IgAN model. This suggests that CS-TPP-EPO nanoparticles may be a potential therapeutic drug for the treatment of patients with IgAN.
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spelling pubmed-40436012014-06-12 Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model ZHANG, XIAOLI WU, YIN SUN, KUN TAN, JING Exp Ther Med Articles The aim of the present study was to investigate the effect of erythropoietin (EPO) loading chitosan-tripolyphosphate (CS-TPP) nanoparticles on an immunoglobulin A nephropathy (IgAN) rat model. CS-TPP nanoparticles were produced from CS and TPP and EPO was loaded by mixing with the nanoparticles. The IgAN rat models were randomly divided into three groups: the CS-TPP-EPO group, CS-TPP group and EPO group. Hemoglobin (Hb), blood urea nitrogen (BUN) and creatinine (Cr) levels were measured in each group using a Biochemical Analyzer (Hitachi, Tokyo, Japan). The average size of nanoparticles was 485±12 nm and the encapsulation efficiency of EPO was 78.45%. The EPO release curve in CS-TPP-EPO nanoparticles exhibited a biphasic distribution in vitro. The levels of BUN and Cr in the CS-TPP-EPO group were significantly lower compared with the control group (P<0.05); however, the level of Hb in the CS-TPP-EPO group was higher compared with the other groups (P<0.05). The changes in Hb, BUN and Cr in the CS-TPP-EPO group were maintained for less than one week following the end of the treatment with CS-TPP-EPO nanoparticles. In conclusion, the CS-TPP-EPO nanoparticles had a lower toxicity compared with EPO and CS-TPP treatment. Furthermore, CS-TPP-EPO may improve the therapeutic effect in the IgAN model. This suggests that CS-TPP-EPO nanoparticles may be a potential therapeutic drug for the treatment of patients with IgAN. D.A. Spandidos 2014-06 2014-03-28 /pmc/articles/PMC4043601/ /pubmed/24926362 http://dx.doi.org/10.3892/etm.2014.1643 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHANG, XIAOLI
WU, YIN
SUN, KUN
TAN, JING
Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title_full Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title_fullStr Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title_full_unstemmed Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title_short Effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an IgA nephropathy rat model
title_sort effect of erythropoietin loading chitosan-tripolyphosphate nanoparticles on an iga nephropathy rat model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043601/
https://www.ncbi.nlm.nih.gov/pubmed/24926362
http://dx.doi.org/10.3892/etm.2014.1643
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