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BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer

Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like...

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Autores principales: Li, Chao, Ji, Yang, Wang, Can, Liang, Shujing, Pan, Fei, Zhang, Chunlei, Chen, Feng, Fu, Hualin, Wang, Kan, Cui, Daxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043660/
https://www.ncbi.nlm.nih.gov/pubmed/24940175
http://dx.doi.org/10.1186/1556-276X-9-244
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author Li, Chao
Ji, Yang
Wang, Can
Liang, Shujing
Pan, Fei
Zhang, Chunlei
Chen, Feng
Fu, Hualin
Wang, Kan
Cui, Daxiang
author_facet Li, Chao
Ji, Yang
Wang, Can
Liang, Shujing
Pan, Fei
Zhang, Chunlei
Chen, Feng
Fu, Hualin
Wang, Kan
Cui, Daxiang
author_sort Li, Chao
collection PubMed
description Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like alkyl chains and multiple carboxyl groups, and then used the prepared amphiphilic polymer to modify QDs. The resultant amphiphilic polymer engineered QDs (PQDs) were conjugated with BRCAA1 and Her2 monoclonal antibody, and prepared BRCAA1 antibody- and Her2 antibody-conjugated QDs were used for in vitro MGC803 cell labeling and in vivo targeted imaging of gastric cancer cells. Results showed that the PQDs exhibited good water solubility, strong photoluminescence (PL) intensity, and good biocompatibility. BRCAA1 antibody- and Her2 antibody-conjugated QD nanoprobes successfully realized targeted imaging of in vivo gastric cancer MGC803 cells. In conclusion, BRCAA1 antibody- and Her2 antibody-conjugated PQDs have great potential in applications such as single cell labeling and in vivo tracking, and targeted imaging and therapeutic effects' evaluation of in vivo early gastric cancer cells in the near future.
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spelling pubmed-40436602014-06-17 BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer Li, Chao Ji, Yang Wang, Can Liang, Shujing Pan, Fei Zhang, Chunlei Chen, Feng Fu, Hualin Wang, Kan Cui, Daxiang Nanoscale Res Lett Nano Express Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like alkyl chains and multiple carboxyl groups, and then used the prepared amphiphilic polymer to modify QDs. The resultant amphiphilic polymer engineered QDs (PQDs) were conjugated with BRCAA1 and Her2 monoclonal antibody, and prepared BRCAA1 antibody- and Her2 antibody-conjugated QDs were used for in vitro MGC803 cell labeling and in vivo targeted imaging of gastric cancer cells. Results showed that the PQDs exhibited good water solubility, strong photoluminescence (PL) intensity, and good biocompatibility. BRCAA1 antibody- and Her2 antibody-conjugated QD nanoprobes successfully realized targeted imaging of in vivo gastric cancer MGC803 cells. In conclusion, BRCAA1 antibody- and Her2 antibody-conjugated PQDs have great potential in applications such as single cell labeling and in vivo tracking, and targeted imaging and therapeutic effects' evaluation of in vivo early gastric cancer cells in the near future. Springer 2014-05-19 /pmc/articles/PMC4043660/ /pubmed/24940175 http://dx.doi.org/10.1186/1556-276X-9-244 Text en Copyright © 2014 Li et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Nano Express
Li, Chao
Ji, Yang
Wang, Can
Liang, Shujing
Pan, Fei
Zhang, Chunlei
Chen, Feng
Fu, Hualin
Wang, Kan
Cui, Daxiang
BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title_full BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title_fullStr BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title_full_unstemmed BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title_short BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer
title_sort brcaa1 antibody- and her2 antibody-conjugated amphiphilic polymer engineered cdse/zns quantum dots for targeted imaging of gastric cancer
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043660/
https://www.ncbi.nlm.nih.gov/pubmed/24940175
http://dx.doi.org/10.1186/1556-276X-9-244
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