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Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF
This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043910/ https://www.ncbi.nlm.nih.gov/pubmed/24893038 http://dx.doi.org/10.1371/journal.pone.0098448 |
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author | Liu, Yang Liu, Yuanyuan Sun, Chao Gan, Lu Zhang, Luwei Mao, Aihong Du, Yuting Zhou, Rong Zhang, Hong |
author_facet | Liu, Yang Liu, Yuanyuan Sun, Chao Gan, Lu Zhang, Luwei Mao, Aihong Du, Yuting Zhou, Rong Zhang, Hong |
author_sort | Liu, Yang |
collection | PubMed |
description | This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditioned medium from cultures of glioma cells irradiated at a range of doses and the migration of both cell types, tube formation by endothelial cells, as well as the expression and secretion of migration-related proteins were evaluated. Exposure to X-ray radiation-conditioned medium induced dose-dependent increases in cell migration and tube formation, which were accompanied by an upregulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and -9 expression. However, glioma cells treated with conditioned medium of cells irradiated at a carbon ion dose of 4.0 Gy showed a marked decrease in migratory potential and VEGF secretion relative to non-irradiated cells. The application of recombinant VEGF165 stimulated migration in glioma and endothelial cells, which was associated with increased FAK phosphorylation at Tyr861, suggesting that the suppression of cell migration by carbon ion radiation could be via VEGF-activated FAK signaling. Taken together, these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment. |
format | Online Article Text |
id | pubmed-4043910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40439102014-06-09 Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF Liu, Yang Liu, Yuanyuan Sun, Chao Gan, Lu Zhang, Luwei Mao, Aihong Du, Yuting Zhou, Rong Zhang, Hong PLoS One Research Article This study evaluated the effects of carbon ion and X-ray radiation and the tumor microenvironment on the migration of glioma and endothelial cells, a key process in tumorigenesis and angiogenesis during cancer progression. C6 glioma and human microvascular endothelial cells were treated with conditioned medium from cultures of glioma cells irradiated at a range of doses and the migration of both cell types, tube formation by endothelial cells, as well as the expression and secretion of migration-related proteins were evaluated. Exposure to X-ray radiation-conditioned medium induced dose-dependent increases in cell migration and tube formation, which were accompanied by an upregulation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and -9 expression. However, glioma cells treated with conditioned medium of cells irradiated at a carbon ion dose of 4.0 Gy showed a marked decrease in migratory potential and VEGF secretion relative to non-irradiated cells. The application of recombinant VEGF165 stimulated migration in glioma and endothelial cells, which was associated with increased FAK phosphorylation at Tyr861, suggesting that the suppression of cell migration by carbon ion radiation could be via VEGF-activated FAK signaling. Taken together, these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment. Public Library of Science 2014-06-03 /pmc/articles/PMC4043910/ /pubmed/24893038 http://dx.doi.org/10.1371/journal.pone.0098448 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Yang Liu, Yuanyuan Sun, Chao Gan, Lu Zhang, Luwei Mao, Aihong Du, Yuting Zhou, Rong Zhang, Hong Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title | Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title_full | Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title_fullStr | Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title_full_unstemmed | Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title_short | Carbon Ion Radiation Inhibits Glioma and Endothelial Cell Migration Induced by Secreted VEGF |
title_sort | carbon ion radiation inhibits glioma and endothelial cell migration induced by secreted vegf |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043910/ https://www.ncbi.nlm.nih.gov/pubmed/24893038 http://dx.doi.org/10.1371/journal.pone.0098448 |
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